Multiple Epitopes from the Mycobacterium tuberculosis ESAT-6 Antigen Are Recognized by Antigen-Specific Human T Cell Lines

A synthetic-peptide approach was used to map epitope regions of the Mycobacterium tuberculosis 6-kDa early secreted antigen target (ESAT-6) by testing human CD4+ T cell lines for secretion of IFN-γ in response to recombinant ESAT-6 (rESAT-6) and overlapping 20-mer peptides covering the antigen seque...

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Bibliographic Details
Published in:	Clinical infectious diseases Vol. 30; no. Supplement-3; pp. S201 - S205
Main Authors:	Mustafa, A. S., Oftung, F., Amoudy, H. A., Madi, N. M., Abal, A. T., Shaban, F., Rosen Krands, I., Andersen, P.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Chicago, IL The University of Chicago Press 01-06-2000 University of Chicago Press
Subjects:	Amino Acid Sequence Antigens Antigens, Bacterial - genetics Antigens, Bacterial - immunology Bacterial diseases Bacterial Proteins Biological and medical sciences CD4-Positive T-Lymphocytes - immunology Cell Line Cell lines Epitope Mapping Epitopes Epitopes, T-Lymphocyte - immunology Histocompatibility Testing Human bacterial diseases Humans Infectious diseases Interferon-gamma - metabolism Lymphocyte Activation Medical sciences Molecular Sequence Data Molecules Mycobacterium tuberculosis Mycobacterium tuberculosis - immunology Peptides - chemical synthesis Peptides - chemistry Peptides - immunology Secretion T lymphocytes Tuberculosis Tuberculosis and atypical mycobacterial infections Tuberculosis vaccine Tuberculosis, Pulmonary - immunology Tuberculosis, Pulmonary - microbiology Vaccination Human Immune response Antigenic determinant HLA-DR-System Vaccination Cytokine Cellular immunity Mycobacterial infection Infection Prevention Cell line Tuberculosis Immunological investigation T-Lymphocyte Bacteriosis Gamma interferon
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Summary:	A synthetic-peptide approach was used to map epitope regions of the Mycobacterium tuberculosis 6-kDa early secreted antigen target (ESAT-6) by testing human CD4+ T cell lines for secretion of IFN-γ in response to recombinant ESAT-6 (rESAT-6) and overlapping 20-mer peptides covering the antigen sequence. The results demonstrate that all of the ESAT-6 peptides screened were able to induce IFN-γ secretion from one or more of the T cell lines tested. Some of the individual T cell lines showed the capacity to respond to all peptides. Human leukocyte antigen (HLA-DR) typing of the donors showed that rESAT-6 was presented to T cells in association with multiple HLA-DR molecules. The results suggest that frequent recognition of the M. tuberculosis ESAT-6 antigen by T cells from patients with tuberculosis is due to the presence of multiple epitopes scattered throughout the ESAT-6 sequence.
Bibliography:	ark:/67375/HXZ-XG3T18TX-R istex:0BC4FCF632F90BC54E06B54F485DF6BEFF43EDEC ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1058-4838 1537-6591
DOI:	10.1086/313862