Search Results - "Ropp, Philip A."

Purification of monoclonal antibodies from clarified cell culture fluid using Protein A capture continuous countercurrent tangential chromatography by Dutta, Amit K., Tran, Travis, Napadensky, Boris, Teella, Achyuta, Brookhart, Gary, Ropp, Philip A., Zhang, Ada W., Tustian, Andrew D., Zydney, Andrew L., Shinkazh, Oleg

“…Recent studies using simple model systems have demonstrated that continuous countercurrent tangential chromatography (CCTC) has the potential to overcome many…”
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Ion chromatographic quantification of cyanate in urea solutions: estimation of the efficiency of cyanate scavengers for use in recombinant protein manufacturing by Lin, Miao-Fang, Williams, Christie, Murray, Michael V., Conn, Greg, Ropp, Philip A.

“…The chaotrope urea is commonly used during recombinant protein manufacturing as a denaturant/solublizing agent. The adventitious accumulation of cyanate in…”
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Characterization of the Native and Recombinant Catalytic Subunit of Human DNA Polymerase γ:  Identification of Residues Critical for Exonuclease Activity and Dideoxynucleotide Sensitivity by Longley, Matthew J, Ropp, Philip A, Lim, Susan E, Copeland, William C

“…The human DNA polymerase γ catalytic subunit was overexpressed in recombinant baculovirus-infected insect cells, and the 136 000 Da protein was purified to…”
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Removal of lipopolysaccharides from protein–lipopolysaccharide complexes by nonflammable solvents by Lin, Miao-Fang, Williams, Christie, Murray, Michael V., Ropp, Philip A.

“…During the recovery of recombinant proteins from gram negative bacteria, many of the methods used to extract proteins from cells release lipopolysaccharides…”
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Cloning and Characterization of the Human Mitochondrial DNA Polymerase, DNA Polymerase γ by Ropp, Philip A., Copeland, William C.

“…The nuclear-encoded DNA polymerase γ (DNA POLγ) is the sole DNA polymerase required for the replication of the mitochondrial DNA. We have cloned the cDNA for…”
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Cloning and Chromosomal Mapping of the Human DNA Polymerase θ (POLQ), the Eighth Human DNA Polymerase by Sharief, Farida S., Vojta, Patrick J., Ropp, Philip A., Copeland, William C.

“…We have cloned the cDNA for the eighth human DNA polymerase, DNA polymerase θ. The human cDNA encodes a putative DNA polymerase of 1762 amino acids with a…”
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Mitochondrial DNA Polymerase γ is Expressed and Translated in the Absence of Mitochondrial DNA Maintenance and Replication by Davis, Alison F., Ropp, Philip A., Clayton, David A., Copeland, William C.

“…Mitochondria are essential organelles in all eukaryotic cells where cellular ATP is generated through the process of oxidative phosphorylation. Protein…”
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DNA polymerase II, the probable homolog of mammalian DNA polymerase epsilon, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae by Araki, H, Ropp, P.A, Johnson, A.L, Johnston, L.H, Morrison, A, Sugino, A

“…Two temperature-sensitive DNA polymerase II mutants (pol2-9 and pol2-18) of the yeast Saccharomyces cerevisiae were isolated by the plasmid shuffling method…”
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Mucin biosynthesis : purification and characterization of a mucin β6N-acetylglucosaminyltransferase by ROPP, P. A, LITTLE, M. R, PI-WAN CHENG

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yeast Saccharomyces cerevisiae DNA polymerase IV: possible involvement in double strand break DNA repair by Leem, S.H, Ropp, P.A, Sugino, A

“…We identified and purified a new DNA polymerase (DNA polymerase IV), which is similar to mammalian DNA polymerase beta, from Saccharomyces cerevisiae and…”
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Characterization of a new DNA polymerase from Schizosaccharomyces pombe: a probable homologue of the Saccharomyces cerevisiae DNA polymerase gamma by Ropp, P A, Copeland, W C

“…Mitochondrial (mt) DNA replication is carried out by the nuclear-encoded DNA polymerase-gamma (Pol-gamma). We have cloned a new DNA polymerase-encoding gene…”
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Purification and characterization of a new DNA polymerase from budding yeast Saccharomyces cerevisiae. A probable homolog of mammalian DNA polymerase beta by Shimizu, K, Santocanale, C, Ropp, P A, Longhese, M P, Plevani, P, Lucchini, G, Sugino, A

“…A new DNA polymerase activity was identified and purified to near homogeneity from extracts of mitotic and meiotic cells of the yeast Saccharomyces cerevisiae…”
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Enzymatic synthesis of UDP-GlcN by a two step hollow fiber enzyme reactor system by Ropp, P A, Cheng, P W

“…UDP-GlcN was synthesized from GlcN and UTP by a two step hollow fiber enzyme reactor method. In step 1, GlcN was converted to GlcN 6-P and then to GlcN 1-P by…”
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