Search Results - "Roosens, Nancy H."

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria by Gand, Mathieu, Bloemen, Bram, Vanneste, Kevin, Roosens, Nancy H C, De Keersmaecker, Sigrid C J

“…Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which improves the reconstruction of genomes and helps to resolve…”
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Current and New Approaches in GMO Detection: Challenges and Solutions by Deforce, Dieter, De Loose, Marc, Taverniers, Isabel, Herman, Philippe, Fraiture, Marie-Alice, Roosens, Nancy H. C.

“…In many countries, genetically modified organisms (GMO) legislations have been established in order to guarantee the traceability of food/feed products on the…”
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Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus by Berbers, Bas, Saltykova, Assia, Garcia-Graells, Cristina, Philipp, Patrick, Arella, Fabrice, Marchal, Kathleen, Winand, Raf, Vanneste, Kevin, Roosens, Nancy H. C., De Keersmaecker, Sigrid C. J.

“…Antimicrobial resistance (AMR) is a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome sequencing (WGS)…”
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Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i by Saltykova, Assia, Wuyts, Véronique, Mattheus, Wesley, Bertrand, Sophie, Roosens, Nancy H C, Marchal, Kathleen, De Keersmaecker, Sigrid C J

“…Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. Besides the technological advances which have pushed the…”
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Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study by Vanneste, Kevin, Garlant, Linda, Broeders, Sylvia, Van Gucht, Steven, Roosens, Nancy H

“…Viral infection by dengue virus is a major public health problem in tropical countries. Early diagnosis and detection are increasingly based on quantitative…”
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Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and antimicrobial resistance surveillance by Bloemen, Bram, Gand, Mathieu, Vanneste, Kevin, Marchal, Kathleen, Roosens, Nancy H. C., De Keersmaecker, Sigrid C. J.

“…Rapid, accurate and comprehensive diagnostics are essential for outbreak prevention and pathogen surveillance. Real-time, on-site metagenomics on miniaturized…”
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Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates by Nouws, Stéphanie, Bogaerts, Bert, Verhaegen, Bavo, Denayer, Sarah, Piérard, Denis, Marchal, Kathleen, Roosens, Nancy H. C., Vanneste, Kevin, De Keersmaecker, Sigrid C. J.

“…Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate characterization and relatedness determination. However, standardized…”
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A Bioinformatics Whole-Genome Sequencing Workflow for Clinical Mycobacterium tuberculosis Complex Isolate Analysis, Validated Using a Reference Collection Extensively Characterized with Conventional Methods and In Silico Approaches by Bogaerts, Bert, Delcourt, Thomas, Soetaert, Karine, Boarbi, Samira, Ceyssens, Pieter-Jan, Winand, Raf, Van Braekel, Julien, De Keersmaecker, Sigrid C J, Roosens, Nancy H C, Marchal, Kathleen, Mathys, Vanessa, Vanneste, Kevin

“…The use of whole-genome sequencing (WGS) for routine typing of bacterial isolates has increased substantially in recent years. For (MTB), in particular, WGS…”
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A systematic review and meta-analysis of host genetic factors associated with influenza severity by Van Goethem, Nina, Danwang, Célestin, Bossuyt, Nathalie, Van Oyen, Herman, Roosens, Nancy H C, Robert, Annie

“…The severity of influenza disease can range from mild symptoms to severe respiratory failure and can partly be explained by host genetic factors that…”
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Status and potential of bacterial genomics for public health practice: a scoping review by Van Goethem, Nina, Descamps, Tine, Devleesschauwer, Brecht, Roosens, Nancy H C, Boon, Nele A M, Van Oyen, Herman, Robert, Annie

“…Next-generation sequencing (NGS) is increasingly being translated into routine public health practice, affecting the surveillance and control of many…”
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Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium by Van Driessche, Laura, Vanneste, Kevin, Bogaerts, Bert, De Keersmaecker, Sigrid C J, Roosens, Nancy H, Haesebrouck, Freddy, De Cremer, Lieze, Deprez, Piet, Pardon, Bart, Boyen, Filip

“…Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G…”
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Are antimicrobial resistance genes key targets to detect genetically modified microorganisms in fermentation products? by Fraiture, Marie-Alice, Deckers, Marie, Papazova, Nina, Roosens, Nancy H.C.

“…As genetically modified microorganisms (GMM), commonly used by the food and feed industry to produce additives, enzymes and flavourings, are frequently…”
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Development of a multiplex mass spectrometry method for simultaneous quantification of urinary proteins related to respiratory health by Nauwelaerts, Sarah J. D., Roosens, Nancy H. C., Bernard, Alfred, De Keersmaecker, Sigrid C. J., De Cremer, Koen

“…Respiratory health of children is a health priority. Club cell protein (CC16) is an interesting biomarker of lung diseases and adverse effects towards the…”
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Sheeppox virus genome sequences from the European outbreaks in Spain, Bulgaria, and Greece in 2022–2024 by Breman, Floris C., Haegeman, Andy, Philips, Wannes, Krešić, Nina, Hoffman, Stefan, De Keersmaecker, Sigrid C. J., Roosens, Nancy H. C., Agüero, Montserrat, Villalba, Ruben, Miteva, Aleksandra, Ivanova, Emiliya, Tasioudi, Konstantia E., Chaintoutis, Serafeim C., Kirtzalidou, Aikaterini, De Regge, Nick

“…In 2022-2024, three outbreaks of sheeppox (SPP) were reported in the European Union. These occurred in Spain, Bulgaria, and Greece and had serious economic…”
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Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants by Van Poelvoorde, Laura A E, Dufrasne, François E, Van Gucht, Steven, Saelens, Xavier, Roosens, Nancy H C

“…The monitoring of antiviral-resistant influenza virus strains is important for public health given the availability and use of neuraminidase inhibitors and…”
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Coverage of the national surveillance system for human Salmonella infections, Belgium, 2016-2020 by Van Goethem, Nina, Van Den Bossche, An, Ceyssens, Pieter-Jan, Lajot, Adrien, Coucke, Wim, Vernelen, Kris, Roosens, Nancy H C, De Keersmaecker, Sigrid C J, Van Cauteren, Dieter, Mattheus, Wesley

“…The surveillance of human salmonellosis in Belgium is dependent on the referral of human Salmonella isolates to the National Reference Center (NRC). Knowledge…”
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Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing by Fraiture, Marie-Alice, Bogaerts, Bert, Winand, Raf, Deckers, Marie, Papazova, Nina, Vanneste, Kevin, De Keersmaecker, Sigrid C. J., Roosens, Nancy H. C.

“…Recently, the unexpected presence of a viable unauthorized genetically modified bacterium in a commercialized food enzyme (protease) product originating from a…”
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Benchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities by Van Uffelen, Alexander, Posadas, Andrés, Roosens, Nancy H. C., Marchal, Kathleen, De Keersmaecker, Sigrid C. J., Vanneste, Kevin

“…Taxonomic classification is crucial in identifying organisms within diverse microbial communities when using metagenomics shotgun sequencing. While…”
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Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking by Fraiture, Marie-Alice, Gobbo, Andrea, Marchesi, Ugo, Verginelli, Daniela, Papazova, Nina, Roosens, Nancy H.C.

“…A PCR-based DNA walking analysis was performed on a protease product suspected to contain a new unauthorized genetically modified microorganism (GMM). Though…”
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MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability by Wuyts, Véronique, Mattheus, Wesley, De Laminne de Bex, Guillaume, Wildemauwe, Christa, Roosens, Nancy H C, Marchal, Kathleen, De Keersmaecker, Sigrid C J, Bertrand, Sophie

“…Surveillance of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is generally considered to benefit from molecular techniques like…”
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