Guidelines for best practice in placebo-controlled experimental studies on probiotics in rodent animal models

In the absence of established best practice standards in the probiotic field for reducing the risk of bacterial transfer between experimental groups, we developed protocols and methods to ensure the highest quality and interpretability of results from animal studies, even when performed in non-conve...

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Bibliographic Details
Published in:	Beneficial microbes Vol. 11; no. 3; p. 245
Main Authors:	Myles, E M, O'Leary, M E, Romkey, I D, Piano, A, de Carvalho, V, Tompkins, T A, Perrot, T S
Format:	Journal Article
Language:	English
Published:	Netherlands 11-05-2020
Subjects:	Animal Experimentation Animals Diarrhea - microbiology Laboratory Animal Science - standards Models, Animal Probiotics - administration & dosage Rodentia - microbiology microbiota methods best practice cross-contamination probiotics
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Summary:	In the absence of established best practice standards in the probiotic field for reducing the risk of bacterial transfer between experimental groups, we developed protocols and methods to ensure the highest quality and interpretability of results from animal studies, even when performed in non-conventional animal care facilities. We describe easily implementable methods for reducing cross-contamination during animal housing, behavioural testing, and euthanasia, along with highlighting protocols for contamination detection in experimental subjects and laboratory areas using qPCR. In light of the high cross-contamination risks between animals during experiments involving probiotics, constant vigilance in animal care and research protocols is critical to ensure valid and reliable research findings.
ISSN:	1876-2891
DOI:	10.3920/BM2019.0144