Semen quality and sperm function loss by hypercholesterolemic diet was recovered by addition of olive oil to diet in rabbit

Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes we...

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Bibliographic Details
Published in:	PloS one Vol. 8; no. 1; p. e52386
Main Authors:	Saez Lancellotti, Tania E, Boarelli, Paola V, Romero, Aida A, Funes, Abi K, Cid-Barria, Macarena, Cabrillana, María E, Monclus, María A, Simón, Layla, Vicenti, Amanda E, Fornés, Miguel W
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 11-01-2013 Public Library of Science (PLoS)
Subjects:	Abnormalities Acrosome Reaction - drug effects Animals Biology Body weight Capacitation Cardiovascular disease Cholesterol Cholesterol, Dietary - administration & dosage Cholesterol, Dietary - adverse effects Diet Diet, High-Fat - adverse effects Dietary Fats, Unsaturated - administration & dosage Dietary Fats, Unsaturated - pharmacology Electrophoresis, Polyacrylamide Gel Fertility High fat diet Hypercholesterolemia Hypercholesterolemia - etiology Hypercholesterolemia - physiopathology Hypercholesterolemia - prevention & control Infertility Laboratory animals Lipids Male Males Medicine Oils & fats Olive Oil Phosphorylation Phosphorylation - drug effects Phosphotyrosine - metabolism Plant Oils - administration & dosage Plant Oils - pharmacology Proteins Rabbits Rodents Seeds Semen Semen Analysis Signal transduction Sperm Sperm Capacitation - drug effects Sperm Count Sperm Motility - drug effects Spermatozoa - drug effects Spermatozoa - physiology Studies Veterinary Science Argentina
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Summary:	Fat increment (0.05% cholesterol, chol) in standard diet promoted a significant increase in serum and sperm membrane chol, which ultimately altered membrane-coupled sperm specific functions: osmotic resistance, acrosomal reaction, and sperm capacitation in White New Zealand rabbits. These changes were also associated with a reduction in motility percentage and appearance of abnormal sperm morphology. The present study was carried out to evaluate the effect of dietary olive oil (OO, 7% v/w) administration to several male hypercholesterolemic rabbits (hypercholesterolemic rabbits, HCR) with altered fertility parameters. These HCR males were achieved by feeding normal rabbits with a high-fat diet (0.05% chol). HCR were associated with a modest non-significant increase in body weight (standard diet, 4.08±0.17 Kg, versus high-fat diet, 4.37±0.24 Kg). Hypercholesterolemic rabbits presented a marked decrease in semen volume, sperm cell count, and percentage of sperm motility, associated with a significant increase in sperm cell abnormalities. Moreover, sperm capacitation measured by the characteristic phosphorylated protein pattern in and induced acrosomal reaction were also altered suggesting sperm dysfunction. However, the administration of OO (for 16 weeks) to rabbits that were fed with 50% of the high-fat diet normalized serum chol. Curiously, OO supply succeeded to attenuate the seminal and sperm alterations observed in HCR group. Administration of OO alone did not cause any significant changes in above mentioned parameters. These data suggest that OO administration to HCR male rabbits recovers the loss of semen quality and sperm functionality.
Bibliography:	Conceived and designed the experiments: TESL PVB MWF. Performed the experiments: TESL PVB AAR AKF MC MEC MAM LS AEV. Analyzed the data: TESL PVB MAM MEC AAR AKF LS MWF. Contributed reagents/materials/analysis tools: MAM AEV MEC. Wrote the paper: TESL MWF. Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0052386