Search Results - "Romano, Louis J."

Single-molecule measurements of synthesis by DNA polymerase with base-pair resolution by Christian, Thomas D, Romano, Louis J, Rueda, David

“…The catalytic mechanism of DNA polymerases involves multiple steps that precede and follow the transfer of a nucleotide to the 3'-hydroxyl of the growing DNA…”
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Crystal Structures of 2-Acetylaminofluorene and 2-Aminofluorene in Complex with T7 DNA Polymerase Reveal Mechanisms of Mutagenesis by Dutta, Shuchismita, Li, Ying, Johnson, Donald, Dzantiev, Leonid, Richardson, Charles C., Romano, Louis J., Ellenberger, Tom

“…The carcinogen 2-acetylaminofluorene forms two major DNA adducts: N-(2′-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and its deacetylated derivative,…”
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Influence of DNA Lesions on Polymerase-Mediated DNA Replication at Single-Molecule Resolution by Gahlon, Hailey L, Romano, Louis J, Rueda, David

“…Faithful replication of DNA is a critical aspect in maintaining genome integrity. DNA polymerases are responsible for replicating DNA, and high-fidelity…”
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Mechanism of aromatic amine carcinogen bypass by the Y-family polymerase, Dpo4 by Brenlla, Alfonso, Rueda, David, Romano, Louis J

“…Bulky DNA damage inhibits DNA synthesis by replicative polymerases and often requires the action of error prone bypass polymerases. The exact mechanism…”
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Single-molecule microscopy reveals new insights into nucleotide selection by DNA polymerase I by Markiewicz, Radoslaw P, Vrtis, Kyle B, Rueda, David, Romano, Louis J

“…The mechanism by which DNA polymerases achieve their extraordinary accuracy has been intensely studied because of the linkage between this process and…”
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Nucleotide selection by the Y-family DNA polymerase Dpo4 involves template translocation and misalignment by Brenlla, Alfonso, Markiewicz, Radoslaw P, Rueda, David, Romano, Louis J

“…Y-family DNA polymerases play a crucial role in translesion DNA synthesis. Here, we have characterized the binding kinetics and conformational dynamics of the…”
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DNA Polymerase: Structural Homology, Conformational Dynamics, and the Effects of Carcinogenic DNA Adducts by Federley, Richard G., Romano, Louis J.

“…DNA replication is vital for an organism to proliferate and lying at the heart of this process is the enzyme DNA polymerase. Most DNA polymerases have a…”
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Carcinogenic adducts induce distinct DNA polymerase binding orientations by Vrtis, Kyle B, Markiewicz, Radoslaw P, Romano, Louis J, Rueda, David

“…DNA polymerases must accurately replicate DNA to maintain genome integrity. Carcinogenic adducts, such as 2-aminofluorene (AF) and N-acetyl-2-aminofluorene…”
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Bulky Lesion Bypass Requires Dpo4 Binding in Distinct Conformations by Liyanage, Pramodha S., Walker, Alice R., Brenlla, Alfonso, Cisneros, G. Andrés, Romano, Louis J., Rueda, David

“…Translesion DNA synthesis is an essential process that helps resume DNA replication at forks stalled near bulky adducts on the DNA. Benzo[ a ]pyrene (B[ a ]P)…”
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Effect of N-2-Acetylaminofluorene and 2-Aminofluorene Adducts on DNA Binding and Synthesis by Yeast DNA Polymerase η by Vooradi, Venkataramana, Romano, Louis J

“…The well-studied aromatic amine carcinogen, N-2-acetylaminofluorene (AAF), forms adducts at the C8 position of guanine in DNA. Unlike replicative polymerases,…”
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Effects of Benzo[a]pyrene Adduct Stereochemistry on Downstream DNA Replication in Vitro:  Evidence for Different Adduct Conformations within the Active Site of DNA Polymerase I (Klenow Fragment) by Alekseyev, Yuriy O, Romano, Louis J

“…The presence of bulky adducts in DNA is known to interfere with DNA replication not only at the site of the lesion but also at positions up to 5 nucleotides…”
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Significance of Nucleobase Shape Complementarity and Hydrogen Bonding in the Formation and Stability of the Closed Polymerase−DNA Complex by Dzantiev, Leonid, Alekseyev, Yuriy O, Morales, Juan C, Kool, Eric T, Romano, Louis J

“…DNA polymerases insert a dNTP by a multistep mechanism that involves a conformational rearrangement from an open to a closed ternary complex, a process that…”
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Monitoring the Conformation of Benzo[a]pyrene Adducts in the Polymerase Active Site Using Fluorescence Resonance Energy Transfer by Christian, Thomas D, Romano, Louis J

“…Benzo[a]pyrene (B[a]P) is a potent environmental carcinogen that is metabolized into diol epoxides that react with exocyclic amines in DNA. These DNA adducts…”
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Benzo[a]pyrene−DNA Adducts Inhibit the DNA Helicase Activity of the Bacteriophage T7 Gene 4 Protein by Yong, Yongqi, Romano, Louis J

“…The gene 4 protein of bacteriophage T7 provides the essential helicase and primase activities for the replication of the T7 genome. In addition, it also…”
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The Role of Specific Amino Acid Residues in the Active Site of Escherichia coli DNA Polymerase I on Translesion DNA Synthesis Across from and Past an N-2-Aminofluorene Adduct by Lone, Samer, Romano, Louis J

“…Understanding how carcinogenic DNA adducts compromise accurate DNA replication is an important goal in cancer research. A central part of these studies is to…”
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Mechanism for N-Acetyl-2-aminofluorene-Induced Frameshift Mutagenesis by Escherichia coli DNA Polymerase I (Klenow Fragment) by Gill, Joshua P, Romano, Louis J

“…N-Acetyl-2-aminofluorene (AAF) is a chemical carcinogen that reacts with guanines at the C8 position in DNA to form a structure that interferes with DNA…”
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Cytosine Methylation in a CpG Sequence Leads to Enhanced Reactivity with Benzo[a]pyrene Diol Epoxide That Correlates with a Conformational Change by Weisenberger, Daniel J., Romano, Louis J.

“…Benzo[a]pyrene (B[a]P) is a widespread environmental carcinogen that must be activated by cellular metabolism to a diol epoxide form (BPDE) before it reacts…”
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Mechanistic Insights into Replication Across from Bulky DNA Adducts:  A Mutant Polymerase I Allows an N-Acetyl-2-aminofluorene Adduct To Be Accommodated during DNA Synthesis by Lone, Samer, Romano, Louis J

“…The molecular mechanism that allows a polymerase to incorporate a nucleotide opposite a DNA lesion is not well-understood. One way to study this process is to…”
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A Conformational Change in E. coli DNA Polymerase I (Klenow Fragment) Is Induced in the Presence of a dNTP Complementary to the Template Base in the Active Site by Dzantiev, Leonid, Romano, Louis J

“…It is well established that the insertion of a nucleotide into a growing DNA chain requires a conformational change in the structure of a DNA polymerase. These…”
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In Vitro Replication of Primer-Templates Containing Benzo[a]pyrene Adducts by Exonuclease-Deficient Escherichia coli DNA Polymerase I (Klenow Fragment):  Effect of Sequence Context on Lesion Bypass by Alekseyev, Yuriy O, Romano, Louis J

“…The presence of benzo[a]pyrene diol epoxide (B[a]PDE) adducts in DNA is known to interfere with DNA replication. Kinetic studies of nucleotide insertion by…”
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