Xenobiotic perturbation of microbial growth as measured by CO2 uptake in aquatic heterotrophic bacteria [Kepone]
A microbiological assay was developed to monitor changes in the growth of aquatic heterotrophic bacteria in the laboratory and in natural waters. This assay, based on the Wood and Werkman reaction (dark reaction carboxylation by heterotrophic bacteria), was used to monitor changes in biosynthetic ac...
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| Published in: | Journal of Great Lakes research Vol. 10; no. 3; pp. 245 - 250 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
1984
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | A microbiological assay was developed to monitor changes in the growth of aquatic heterotrophic bacteria in the laboratory and in natural waters. This assay, based on the Wood and Werkman reaction (dark reaction carboxylation by heterotrophic bacteria), was used to monitor changes in biosynthetic activity of heterotrophic bacteria stimulated with an organic nutrient and incubated with aquatic chemical contaminants. Bacterial carboxylation and growth was significantly inhibited by kepone at water concentrations greater than 1.0 mg per litre; There was a direct dose-response relationship at concentrations from 1.0-10.0 mg per litre. Simazine slightly stimulated carbon dioxide fixation by bacteria. Neither di-2-ethylhexyl phthalate (DEHP), Dylox, nor mirex affected heterotrophic carboxylation. |
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| Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| ISSN: | 0380-1330 |
| DOI: | 10.1016/S0380-1330(84)71835-1 |