Search Results - "Rollet, Kévin"
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Nucleating Agent Crystallophore Induces Instant Protein Crystallization
Published in Crystal growth & design (21-08-2024)“…The rapid preparation of homogeneous suspensions of micro- or nanocrystals is a crucial step in serial crystallography. We show how additives, such as the…”
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Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA Substrates
Published in International journal of molecular sciences (28-11-2020)“…The mitochondrial genome of the nematode encodes for miniaturized hairpin-like tRNA molecules that lack D- as well as T-arms, strongly deviating from the…”
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H1153Y-KCNH2 Mutation Identified in a Sudden Arrhythmic Death Syndrome Case Alters Channel Gating
Published in International journal of molecular sciences (26-08-2021)“…Long QT syndrome is one of the most common hereditary channelopathies inducing fatal arrhythmias and sudden cardiac death. We identified in a sudden arrhythmic…”
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CCA-addition in the cold: Structural characterization of the psychrophilic CCA-adding enzyme from the permafrost bacterium Planococcus halocryophilus
Published in Computational and structural biotechnology journal (01-01-2021)“…[Display omitted] •A high-resolution structure of a psychrophilic RNA polymerase contributes to our knowledge of cold adaptation.•While catalytic core motifs…”
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Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Published in Journal of visualized experiments (20-03-2021)“…The preparation of well diffracting crystals and their handling before their X-ray analysis are two critical steps of biocrystallographic studies. We describe…”
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Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering
Published in Crystals (Basel) (01-02-2020)“…The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called…”
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Structural Analysis of RNA by Small-Angle X-ray Scattering
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2020)“…Over the past two decades small-angle X-ray scattering (SAXS) has become a popular method to characterize solutions of biomolecules including ribonucleic acid…”
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A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
Published in IUCrJ (01-05-2019)“…Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device…”
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Monitoring the crystallization of two enzymes in real time by dynamic light scattering
Published in Acta crystallographica. Section A, Foundations and advances (14-08-2021)“…Abstract only…”
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