Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent

Ampicillin has been shown to improve glucose tolerance in mice. We hypothesized that this effect is present only if treatment is initiated prior to weaning and that it disappears when treatment is terminated. High-fat fed C57BL/6NTac mice were divided into groups that received Ampicillin at differen...

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Bibliographic Details
Published in:Journal of diabetes research Vol. 2013; no. 2013; pp. 1 - 13
Main Authors: Rune, I., Hansen, C. H. F., Ellekilde, M., Nielsen, D. S., Skovgaard, K., Rolin, B. C., Lykkesfeldt, J., Josefsen, K., Tranberg, B., Kihl, P., Hansen, A. K.
Format: Journal Article
Language:English
Published: Cairo, Egypt Hindawi Puplishing Corporation 01-01-2013
Hindawi Publishing Corporation
Hindawi Limited
Subjects:
Age
Ampicillin - therapeutic use
Animals
Antibiotics
Bacteria
Blood Glucose - drug effects
Chromium
Cytokines
Dendritic Cells - drug effects
Dendritic Cells - pathology
Diabetes
Diet
Diet, High-Fat
Disease
Gastrointestinal Tract - drug effects
Gastrointestinal Tract - microbiology
Gene expression
Glucose
Glucose Intolerance - blood
Glucose Intolerance - immunology
Glucose Intolerance - prevention & control
Glucose Tolerance Test
Gram-negative bacteria
Gram-positive bacteria
Inflammation
Insulin resistance
Metabolism
Mice
Mice, Inbred C57BL
Mice, Obese
Microbiota
Microbiota - drug effects
Obesity - blood
Obesity - drug therapy
Obesity - etiology
Obesity - immunology
Penicillin
Studies
T-Lymphocytes, Regulatory - drug effects
T-Lymphocytes, Regulatory - pathology
Tumor necrosis factor-TNF
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Summary:Ampicillin has been shown to improve glucose tolerance in mice. We hypothesized that this effect is present only if treatment is initiated prior to weaning and that it disappears when treatment is terminated. High-fat fed C57BL/6NTac mice were divided into groups that received Ampicillin at different ages or not at all. We found that both diet and Ampicillin significantly changed the gut microbiota composition in the animals. Furthermore, there was a significant improvement in glucose tolerance in Ampicillin-treated, five-week-old mice compared to nontreated mice in the control group. At study termination, expressions of mRNA coding for tumor necrosis factor, serum amyloid A, and lactase were upregulated, while the expression of tumor necrosis factor (ligand) superfamily member 15 was downregulated in the ileum of Ampicillin-treated mice. Higher dendritic cell percentages were found systemically in high-fat diet mice, and a lower tolerogenic dendritic cell percentage was found both in relation to high-fat diet and late Ampicillin treatment. The results support our hypothesis that a “window” exists early in life in which an alteration of the gut microbiota affects glucose tolerance as well as development of gut immunity and that this window may disappear after weaning.
Bibliography:Academic Editor: Toshiyasu Sasaoka
ISSN:2314-6745
2314-6753
DOI:10.1155/2013/319321