Search Results - "Roldão, Antonio"

Efficacies of insecticide dog collars against visceral leishmaniasis in low and high-income areas and the effects for non-collared neighbor dogs by Matsumoto, Patricia Sayuri Silvestre, Taniguchi, Helena Hilomi, Pereira, Virgínia Bodelão Richini, Hiramoto, Roberto Mitsuyoshi, Seviero Rampazzi, Karla Letícia, de Raeffray Barbosa, José Eduardo, Puci Neto, Roldão Antonio, Camprigher, Valéria Medina, de Barros Cortez, Luiz Ricardo Paes, Rahaman, Khan Rubayet, Novak, Mathew, Tolezano, José Eduardo

“…•4% Deltamethrin-Impregnated Collared (DMC) dogs were successfully protected (63%) against canine visceral leishmaniasis (CVL) compared to control dogs.•DMC…”
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Functionalizing Ferritin Nanoparticles for Vaccine Development by Rodrigues, Margarida Q., Alves, Paula M., Roldão, António

“…In the last decade, the interest in ferritin-based vaccines has been increasing due to their safety and immunogenicity. Candidates against a wide range of…”
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Tyrosinase-Mediated Conjugation for Antigen Display on Ferritin Nanoparticles by Rodrigues, Margarida Q., Patão, Sara, Thomaz, Mónica, Nunes, Tiago, Alves, Paula M., Roldão, António

“…Ferritin (Ft) nanoparticles have become versatile platforms for displaying antigens, being a promising technology for vaccine development. While genetic fusion…”
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Synthetic biology for bioengineering virus‐like particle vaccines by Charlton Hume, Hayley K., Vidigal, João, Carrondo, Manuel J. T., Middelberg, Anton P. J., Roldão, António, Lua, Linda H. L.

“…Vaccination is the most effective method of disease prevention and control. Many viruses and bacteria that once caused catastrophic pandemics (e.g., smallpox,…”
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Advanced cell technologies: Making protein, cell, and gene therapies a reality by Geada, Isabelle, Roldão, Antonio, Betenbaugh, Michael J., Alves, Paula M.

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Large-scale production and purification of VLP-based vaccines by Vicente, Tiago, Roldão, António, Peixoto, Cristina, Carrondo, Manuel J.T., Alves, Paula M.

“…Relevant strategies implemented to overcome the challenges of large-scale production (USP) and purification (DSP) of VLP-vaccines using the BEVS/IC. These…”
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Virus-like particles in vaccine development by Roldão, António, Mellado, Maria Candida M, Castilho, Leda R, Carrondo, Manuel J T, Alves, Paula M

“…Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome,…”
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Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production by Fernandes, Bárbara, Vidigal, João, Correia, Ricardo, Carrondo, Manuel J.T., Alves, Paula M., Teixeira, Ana P., Roldão, António

“…•Adaptive laboratory evolution (ALE) of stable insect cell lines to hypothermic culture conditions.•Adapted cells express up to 26-fold more Gag-VLPs than…”
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Process intensification for Peste des Petites Ruminants Virus vaccine production by Sousa, Marcos, Fenge, Christel, Rupprecht, Jens, Tappe, Alexander, Greller, Gerhard, Alves, Paula, Carrondo, Manuel, Roldão, António

“…•Process intensification for PPRV vaccine production in Vero cells.•Agitation requirements for microcarrier-based bioreactor cultures and scale-up.•Improved…”
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Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes by Sousa, Marcos F. Q., Silva, Marta M., Giroux, Daniel, Hashimura, Yas, Wesselschmidt, Robin, Lee, Brian, Roldão, António, Carrondo, Manuel J. T., Alves, Paula M., Serra, Margarida

“…Anchorage‐dependent cell cultures are used for the production of viruses, viral vectors, and vaccines, as well as for various cell therapies and tissue…”
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Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq by Virgolini, Nikolaus, Silvano, Marco, Hagan, Ryan, Correia, Ricardo, Alves, Paula M., Clarke, Colin, Roldão, António, Isidro, Inês A.

“…The insect cell‐baculovirus expression vector system (IC‐BEVS) has shown to be a powerful platform to produce complex biopharmaceutical products, such as…”
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Towards a scalable bioprocess for rAAV production using a HeLa stable cell line by Escandell, José, Moura, Filipa, Carvalho, Sofia B., Silva, Ricardo J. S., Correia, Ricardo, Roldão, Antonio, Gomes‐Alves, Patrícia, Alves, Paula M.

“…The majority of recombinant adeno‐associated viruses (rAAV) approved for clinical use or in clinical trials areproduced by transient transfection using the…”
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A novel asexual blood-stage malaria vaccine candidate: PfRipr5 formulated with human-use adjuvants induces potent growth inhibitory antibodies by Takashima, Eizo, Nagaoka, Hikaru, Correia, Ricardo, Alves, Paula M, Roldão, António, Christensen, Dennis, Guderian, Jeffrey A, Fukushima, Akihisa, Viebig, Nicola K, Depraetere, Hilde, Tsuboi, Takafumi

“…PfRipr is a highly conserved asexual-blood stage malaria vaccine candidate against . PfRipr5, a protein fragment of PfRipr inducing the most potent inhibitory…”
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Integrating high cell density cultures with adapted laboratory evolution for improved Gag‐HA virus‐like particles production in stable insect cell lines by Fernandes, Bárbara, Correia, Ricardo, Sousa, Marcos, Carrondo, Manuel J. T., Alves, Paula M., Roldão, António

“…Stable insect cell lines are emerging as an alternative to the insect cell‐baculovirus expression vector system (IC‐BEVS) for protein expression, benefiting…”
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Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing by Silvano, Marco, Correia, Ricardo, Virgolini, Nikolaus, Clarke, Colin, Alves, Paula M, Isidro, Inês A, Roldão, António

“…Adaptive laboratory evolution has been used to improve production of influenza hemagglutinin (HA)-displaying virus-like particles (VLPs) in insect cells…”
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Vaccine-induced neutralizing antibody responses to seasonal influenza virus H1N1 strains are not enhanced during subsequent pandemic H1N1 infection by Mooij, Petra, Mortier, Daniella, Aartse, Aafke, Murad, Alexandre B., Correia, Ricardo, Roldão, António, Alves, Paula M., Fagrouch, Zahra, Eggink, Dirk, Stockhofe, Norbert, Engelhardt, Othmar G., Verschoor, Ernst J., van Gils, Marit J., Bogers, Willy M., Carrondo, Manuel J. T., Remarque, Edmond J., Koopman, Gerrit

“…The first exposure to influenza is presumed to shape the B-cell antibody repertoire, leading to preferential enhancement of the initially formed responses…”
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Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature by Fernandes, Bárbara, Correia, Ricardo, Alves, Paula M., Roldão, António

“…Protein production processes based on stable insect cell lines require intensification to be competitive with the insect cell-baculovirus expression vector…”
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Improved storage of influenza HA-VLPs using a trehalose-glycerol natural deep eutectic solvent system by Correia, Ricardo, Meneses, Liane, Richheimer, Carolina, Alves, Paula M., Carrondo, Manuel J.T., Duarte, Ana Rita C., Paiva, Alexandre, Roldão, António

“…Vaccines are typically stored under refrigerated conditions (2–8 °C) to limit potency and efficacy loss. Maintaining the cold chain is costly and prone to…”
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Transcriptome analysis of Sf9 insect cells during production of recombinant Adeno‐associated virus by Virgolini, Nikolaus, Hagan, Ryan, Correia, Ricardo, Silvano, Marco, Fernandes, Sofia, Alves, Paula M., Clarke, Colin, Roldão, António, Isidro, Inês A.

“…The insect cell‐baculovirus expression vector system (IC‐BEVS) has emerged as an alternative time‐ and cost‐efficient production platform for recombinant…”
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Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production by Sequeira, Daniela P, Correia, Ricardo, Carrondo, Manuel J.T, Roldão, António, Teixeira, Ana P, Alves, Paula M

“…Highlights • Combining stable and baculovirus-mediated expression for multi-HA influenza VLPs production. • Modular strategy as competitive as co-infection…”
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