Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep

Evaluation of a modified Rose Bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of Brucella melitensis infection in sheep

A modified Rose Bengal test (mRB) and an indirect ELISA (iELISA) with Protein G as the conjugate, were evaluated for the diagnosis of Brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard Rose Ben...

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Bibliographic Details
Published in:Veterinary research (Paris) Vol. 34; no. 3; p. 297
Main Authors: Ferreira, Ana Cristina, Cardoso, Regina, Travassos Dias, Isabel, Mariano, Isabel, Belo, Ana, Rolão Preto, Isabel, Manteigas, António, Pina Fonseca, António, Corrêa De Sá, Maria Inácia
Format: Journal Article
Language:English
Published: England 01-05-2003
Subjects:
Animals
Brucella melitensis - immunology
Brucella melitensis - isolation & purification
Brucellosis - blood
Brucellosis - diagnosis
Brucellosis - immunology
Brucellosis - veterinary
Enzyme-Linked Immunosorbent Assay - methods
Rose Bengal
Sensitivity and Specificity
Sheep Diseases - blood
Sheep Diseases - diagnosis
Sheep Diseases - immunology
Sheep Diseases - microbiology
Sheep, Domestic - immunology
Sheep, Domestic - microbiology
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Summary:A modified Rose Bengal test (mRB) and an indirect ELISA (iELISA) with Protein G as the conjugate, were evaluated for the diagnosis of Brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard Rose Bengal (RB) and Complement Fixation (CF) tests used in the current eradication campaign in EU countries. All tests showed 100% specificity when testing the sera from 212 Brucella-free sheep. When testing the sera from 219 Brucella melitensis culture-positive sheep, both the mRB and iELISA tests were more sensitive (98.6% and 96.8%, respectively) than the RB and CF tests (95.0% and 92.7%, respectively). These results were similar when testing the sera from 181 animals belonging to infected flocks but found bacteriologically negative, suggesting that the mRB or iELISA tests could advantageously replace the current RB procedure used as the screening test.
ISSN:0928-4249
DOI:10.1051/vetres:2003005