Characterization of BcgI, a new kind of restriction-modification system

The BcgI restriction enzyme from Bacillus coagulans is unusual in that it cleaves on both sides of its recognition site, CGAN6TGC, releasing a fragment that includes the site and several bases on each side. We report the organization and nucleotide sequences of the genes for the BcgI restriction-mod...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 269; no. 1; pp. 683 - 690
Main Authors:	Kong, H, Roemer, S E, Waite-Rees, P A, Benner, J S, Wilson, G G, Nwankwo, D O
Format:	Journal Article
Language:	English
Published:	Bethesda, MD American Society for Biochemistry and Molecular Biology 07-01-1994
Subjects:	Adenine - analogs & derivatives Adenine - biosynthesis Amino Acid Sequence Analytical, structural and metabolic biochemistry Bacillus - enzymology Bacillus coagulans Base Sequence Biological and medical sciences Cloning, Molecular Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - isolation & purification Deoxyribonucleases, Type II Site-Specific - metabolism DNA, Bacterial - metabolism Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genes, Bacterial Hydrolases Methylation Molecular Sequence Data Oligodeoxyribonucleotides Protein Binding Restriction Mapping Sequence Homology, Amino Acid Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics Site-Specific DNA-Methyltransferase (Adenine-Specific) - isolation & purification Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Ligand binding Bacillales Enzymatic activity Enzyme Restriction endonuclease Bacteria Primary structure Bacillaceae Molecular cloning Subunit Bacillus coagulans
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Summary:	The BcgI restriction enzyme from Bacillus coagulans is unusual in that it cleaves on both sides of its recognition site, CGAN6TGC, releasing a fragment that includes the site and several bases on each side. We report the organization and nucleotide sequences of the genes for the BcgI restriction-modification system and the properties of the proteins that they encode. The system comprises two adjacent, similarly oriented genes. The proximal gene, bcgIA, codes for a 637-amino acid protein (molecular mass = 71.6 kDa) that resembles certain m6A-specific DNA-methyltransferases, particularly those that constitute the modification subunits of type I restriction-modification systems. The distal gene, bcgIB, codes for a 341-amino acid protein (molecular mass = 39.2 kDa) that resembles none of the sequences in the sequence data bases. The two genes overlap by several nucleotides. Alone, neither protein restricts or modifies DNA, but, together, they form a complex in the proportion A2B that does both. DNA binding assays showed that the DNA-protein complex can be formed only in the presence of both subunits, suggesting that the association of inactive subunits generates the active BcgI enzyme that can bind DNA and then either cleaves or methylates at target site.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(17)42403-3