Search Results - "Roelofs, Jeroen"

Structures of chaperone-associated assembly intermediates reveal coordinated mechanisms of proteasome biogenesis by Schnell, Helena M., Walsh, Richard M., Rawson, Shaun, Kaur, Mandeep, Bhanu, Meera K., Tian, Geng, Prado, Miguel A., Guerra-Moreno, Angel, Paulo, Joao A., Gygi, Steven P., Roelofs, Jeroen, Finley, Daniel, Hanna, John

“…The proteasome mediates most selective protein degradation. Proteolysis occurs within the 20S core particle (CP), a barrel-shaped chamber with an α 7 β 7 β 7 α…”
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Tagging the proteasome active site β5 causes tag specific phenotypes in yeast by Waite, Kenrick A., Burris, Alicia, Roelofs, Jeroen

“…The efficient and timely degradation of proteins is crucial for many cellular processes and to maintain general proteostasis. The proteasome, a complex…”
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Assembly, structure, and function of the 26S proteasome by Bedford, Lynn, Paine, Simon, Sheppard, Paul W, Mayer, R. John, Roelofs, Jeroen

“…The 26S proteasome is a large multiprotein complex involved in the regulated degradation of ubiquitinated proteins in the cell. The 26S proteasome has been…”
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Proteaphagy and the Trafficking of Proteasomes under Nutrient Stress Conditions by Waite, Kenrick A, Roelofs, Jeroen

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Proteasome inhibition by bortezomib: A left hook and a right punch by Roelofs, Jeroen

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Chaperone-mediated pathway of proteasome regulatory particle assembly by Shi, Yigong, Roelofs, Jeroen, Gygi, Steven P, Zhang, Fan, Haas, Wilhelm, Lee, Byung-Hoon, Finley, Daniel, Tian, Geng, Park, Soyeon, Huo, Ying, McAllister, Fiona E

“…The proteasome is a protease that controls diverse processes in eukaryotic cells. Its regulatory particle (RP) initiates the degradation of ubiquitin-protein…”
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A proteomics approach to understanding protein ubiquitination by Gygi, Steven P, Peng, Junmin, Schwartz, Daniel, Elias, Joshua E, Thoreen, Carson C, Cheng, Dongmei, Marsischky, Gerald, Roelofs, Jeroen, Finley, Daniel

“…There is a growing need for techniques that can identify and characterize protein modifications on a large or global scale. We report here a proteomics…”
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Reconfiguration of the proteasome during chaperone-mediated assembly by Park, Soyeon, Li, Xueming, Kim, Ho Min, Singh, Chingakham Ranjit, Tian, Geng, Hoyt, Martin A., Lovell, Scott, Battaile, Kevin P., Zolkiewski, Michal, Coffino, Philip, Roelofs, Jeroen, Cheng, Yifan, Finley, Daniel

“…The proteasome degrades ubiquitin-conjugated substrates; here, structural and functional insights from studies in yeast reveal that it is reconfigured during…”
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The Proteasome-associated Protein Ecm29 Inhibits Proteasomal ATPase Activity and in Vivo Protein Degradation by the Proteasome by De La Mota-Peynado, Alina, Lee, Stella Yu-Chien, Pierce, Brianne Marie, Wani, Prashant, Singh, Chingakham Ranjit, Roelofs, Jeroen

“…Several proteasome-associated proteins regulate degradation by the 26 S proteasome using the ubiquitin chains that mark most substrates for degradation. The…”
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The Extent of Extended-Ubiquitin Binding to the Proteasome by Roelofs, Jeroen

“…In this issue of Structure, Lu et al. (2020) describe an NMR-based study showing the proteasome ubiquitin receptor hRpn13 bound to an extended conformation of…”
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Cooperativity in Proteasome Core Particle Maturation by Suppahia, Anjana, Itagi, Pushpa, Burris, Alicia, Kim, Faith Mi Ge, Vontz, Alexander, Kante, Anupama, Kim, Seonghoon, Im, Wonpil, Deeds, Eric J., Roelofs, Jeroen

“…Proteasomes are multi-subunit protease complexes found in all domains of life. The maturation of the core particle (CP), which harbors the active sites,…”
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Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia by Postma, Marten, Roelofs, Jeroen, Goedhart, Joachim, Gadella, Theodorus W J, Visser, Antonie J W G, Van Haastert, Peter J M

“…The chemoattractant cAMP induces the translocation of cytosolic PHCrac-GFP to the plasma membrane. PHCrac-GFP is a green fluorescent protein fused to a PH…”
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Reduced Protein Diffusion Rate by Cytoskeleton in Vegetative and Polarized Dictyostelium Cells by Potma, Eric O., Boeij, Wim P. de, Bosgraaf, Leonard, Roelofs, Jeroen, van Haastert, Peter J.M., Wiersma, Douwe A

“…Fluorescence recovery after photobleaching measurements with high spatial resolution are performed to elucidate the impact of the actin cytoskeleton on…”
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1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain by Singh, Chingakham R., Lovell, Scott, Mehzabeen, Nurjahan, Chowdhury, Wasimul Q., Geanes, Eric S., Battaile, Kevin P., Roelofs, Jeroen

“…The 26S proteasome is a 2.5 MDa protease dedicated to the degradation of ubiquitinated proteins in eukaryotes. The assembly of this complex containing 66…”
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Proteasome granule formation is regulated through mitochondrial respiration and kinase signaling by Waite, Kenrick A., Roelofs, Jeroen

“…ABSTRACT In the yeast Saccharomyces cerevisiae, proteasomes are enriched in cell nuclei, in which they execute important cellular functions. Nutrient stress…”
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Proteasome Localization is Regulated Through Mitochondrial Respiration and Kinase Signaling by Waite, Kenrick A., Roelofs, Jeroen

“…In most organisms, proteasomes are enriched in cell nuclei under optimal physiological conditions, indicating an important need for protein degradation in this…”
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Proteaphagy is specifically regulated and requires factors dispensable for general autophagy by Waite, Kenrick A., Burris, Alicia, Vontz, Gabrielle, Lang, Angelica, Roelofs, Jeroen

“…Changing physiological conditions can increase the need for protein degradative capacity in eukaryotic cells. Both the ubiquitin-proteasome system and…”
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Proteasome condensate formation is driven by multivalent interactions with shuttle factors and ubiquitin chains by Waite, Kenrick A, Vontz, Gabrielle, Lee, Stella Y, Roelofs, Jeroen

“…Stress conditions can cause the relocalization of proteasomes to condensates in yeast and mammalian cells. The interactions that facilitate the formation of…”
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Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles by Waite, Kenrick A., Mota-Peynado, Alina De-La, Vontz, Gabrielle, Roelofs, Jeroen

“…The proteasome is responsible for the degradation of many cellular proteins. If and how this abundant and normally stable complex is degraded by cells is…”
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Proteasome Shuttle Factors Regulate the Relocalization of Proteasomes to Cytosolic Granules upon Specific Stress Conditions by Roelofs, Jeroen, Vontz, Gabrielle, Waite, Kenrick A., Lee, Stella Y.

“…Proteasomes are essential complexes within cells responsible for the degradation of many short‐lived and aberrant proteins. These substrates are marked for…”
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