Alleviation of Oxidative Stress Induced by 24-Epibrassinolide in Soybean Plants Exposed to Different Manganese Supplies: UpRegulation of Antioxidant Enzymes and Maintenance of Photosynthetic Pigments

Alleviation of Oxidative Stress Induced by 24-Epibrassinolide in Soybean Plants Exposed to Different Manganese Supplies: UpRegulation of Antioxidant Enzymes and Maintenance of Photosynthetic Pigments

Adverse effects promoted by inadequate manganese (Mn) supply (deficiency or toxicity) causes inefficiency of the antioxidant system and degradation of chlorophylls. However, 24-epibrassinolide (EBR) is a natural steroid that exhibits beneficial effects on antioxidant metabolism, chlorophyll levels a...

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Bibliographic Details
Published in:Journal of plant growth regulation Vol. 39; no. 4; pp. 1425 - 1440
Main Authors: Rodrigues, Wenderson da Silva, Pereira, Ynglety Cascaes, de Souza, André Luiz Marques, Batista, Bruno Lemos, Lobato, Allan Klynger da Silva
Format: Journal Article
Language:English
Published: New York Springer US 01-12-2020
Springer Nature B.V
Subjects:
Agriculture
Antioxidants
Ascorbic acid
Biomedical and Life Sciences
Carotenoids
Catalase
Chlorophyll
Chloroplasts
Electrolyte leakage
Enzymes
Factorial design
Hydrogen peroxide
L-Ascorbate peroxidase
Life Sciences
Maintenance
Malondialdehyde
Manganese
Oxidative stress
Peroxidase
Photosynthesis
Photosynthetic pigments
Pigments
Plant Anatomy/Development
Plant Physiology
Plant Sciences
Soybeans
Steroids
Superoxide dismutase
Supplements
Toxicity
Antioxidant system
Stress indicators
Brassinosteroids
Superoxide
Hydrogen peroxide
Chlorophylls
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Summary:Adverse effects promoted by inadequate manganese (Mn) supply (deficiency or toxicity) causes inefficiency of the antioxidant system and degradation of chlorophylls. However, 24-epibrassinolide (EBR) is a natural steroid that exhibits beneficial effects on antioxidant metabolism, chlorophyll levels and stress indicators. Therefore, this research aims to evaluate whether EBR application via spray can alleviate oxidative stress in soybean plants exposed to different Mn concentrations and to determine possible contributions of the antioxidant enzymes and photosynthetic pigments. Experiment followed a completely randomized factorial design with two concentrations of 24-epibrassinolide (0 and 100 nM EBR, described as − EBR and + EBR, respectively) and three Mn doses (0.25, 25 and 2500 µM Mn, described as low, control and high supply of Mn, respectively). Plants treated with low and high concentrations of Mn + EBR exhibit significant increases in all enzymes evaluated (superoxide dismutase, catalase, ascorbate peroxidase and peroxidase). To superoxide dismutase (SOD), EBR spray promoted increments of 77%, 38% and 76% under low, control and high Mn supplementation, respectively, compared to same treatment in absence of EBR. Clearly intense activity is linked to SOD contributed by dismutation of superoxide into hydrogen peroxide, being subsequently decomposed by other enzymes (catalase, ascorbate peroxidase and peroxidase). Concomitantly, plants with Mn deficiency and toxicity sprayed with 100 nM EBR presented maintenance of chlorophylls and carotenoids due to reduction of superoxide and hydrogen peroxide and consequently reduced chloroplast membrane damages as indicated by malondialdehyde levels and electrolyte leakage.
ISSN:0721-7595
1435-8107
DOI:10.1007/s00344-020-10091-7