Metallothionein gene expression is altered in oral cancer and may predict metastasis and patient outcomes

Aims Metallothioneins (MTs) are proteins associated with the carcinogenesis and prognosis of various tumours. Previous studies have shown their potential as biomarkers in oral squamous cell carcinoma (OSCC). Aiming to understand more clearly the function of MTs in OSCC we evaluated, for the first ti...

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Bibliographic Details
Published in:	Histopathology Vol. 67; no. 3; pp. 358 - 367
Main Authors:	Brazão-Silva, Marco T, Rodrigues, Maria Fernandes S, Eisenberg, Ana Lúcia A, Dias, Fernando L, de Castro, Luciana M, Nunes, Fábio D, Faria, Paulo R, Cardoso, Sérgio V, Loyola, Adriano M, de Sousa, Suzana C O M
Format:	Journal Article
Language:	English
Published:	England Blackwell Publishing Ltd 01-09-2015 Wiley Subscription Services, Inc
Subjects:	Aged Biomarkers, Tumor - genetics Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - secondary Cervical cancer Down-Regulation Female Gene expression Humans Male Matrix Metalloproteinase 16 - genetics metallothionein Metallothionein - genetics Metastasis Middle Aged Mouth Mucosa - enzymology Mouth Neoplasms - genetics Mouth Neoplasms - pathology oral squamous cell carcinoma Prognosis RNA, Messenger - genetics RNA, Neoplasm - genetics RT-qPCR Tongue Neoplasms - genetics Tongue Neoplasms - pathology Up-Regulation oral squamous cell carcinoma metallothionein metastasis RT-qPCR prognosis
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Summary:	Aims Metallothioneins (MTs) are proteins associated with the carcinogenesis and prognosis of various tumours. Previous studies have shown their potential as biomarkers in oral squamous cell carcinoma (OSCC). Aiming to understand more clearly the function of MTs in OSCC we evaluated, for the first time, the gene expression profile of MTs in this neoplasm. Materials and results Tissue samples from 35 cases of tongue and/or floor of mouth OSCC, paired with their corresponding non‐neoplastic oral mucosa (NNOM), were retrieved (2007–09). All tissues were analysed for the following genes using TaqMan® reverse transcription–quantitative polymerase chain reaction (RT–qPCR) assays: MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT3 and MT4. The expression of MT1B and MT1H was seldom detected in both OSCC and NNOM. A significant loss of MT1A, MT1X, MT3 and MT4 expression and gain of MT1F expression was observed in OSCC, compared to NNOM. Cases with MT1G down‐regulation exhibited the worst prognoses. The up‐regulation of MT1X was restricted to non‐metastatic cases, whereas up‐regulation of MT3 was related to cases with lymph node metastasis. Conclusions Metallothionein mRNA expression is altered significantly in oral squamous cell carcinomas. The expression of MT1G, MT1X and MT3 may aid in the prognostic discrimination of OSCC cases.
Bibliography:	CNPq ark:/67375/WNG-P0VMP4P5-3 FAPEMIG ArticleID:HIS12660 istex:013C62C3BC7ED610B57C41AFEB2D9F09C7BF97EC CAPES
ISSN:	0309-0167 1365-2559
DOI:	10.1111/his.12660