Maxillary suture expansion: A mouse model to explore the molecular effects of mechanically-induced bone remodeling

The aim of this study was to analyze the effect of rapid maxillary expansion (RME) on hard tissues. Opening loops bonded to the first and second maxillary molars on both sides were used to apply distracting forces of 0.28 N, 0.42 N and 0.56 N at the midpalatal suture for 7 and 14 days. Microcomputed...

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Bibliographic Details
Published in:	Journal of biomechanics Vol. 108; p. 109880
Main Authors:	Guerrero, Jose Alejandro, Silva, Raquel Souto, de Abreu Lima, Izabella Lucas, Rodrigues, Bianca Cristina Duffles, Barrioni, Breno Rocha, Amaral, Flávio Almeida, Tabanez, André Petenuci, Garlet, Gustavo Pompermaier, Alvarado, Diego Alexander Garzon, Silva, Tarcília Aparecida, de Las Casas, Estevam Barbosa, Macari, Soraia
Format:	Journal Article
Language:	English
Published:	United States Elsevier Ltd 17-07-2020 Elsevier Limited
Subjects:	Alkaline phosphatase Animals Biomarkers Biomedical materials Bone histomorphometry Bone morphogenetic protein 2 Bone morphogenetic proteins Bone Remodeling Bones Cartilage Cathepsin K Cbfa-1 protein Chondrocytes Collagen (type I) Computed tomography Dentin Endodontics Experimental model Gelatinase B Markers Matrix metalloproteinase Matrix metalloproteinases Maxilla Maxillary suture expansion Mice Molars Molecular analysis Orthodontics Osteoblasts Osteocalcin Osteoclasts Osteoprotegerin Osteoprotegerin - genetics Palatal Expansion Technique Polymerase chain reaction Proteins RANK Ligand Receptors Reproducibility of Results SOST protein Sutures Teeth TRANCE protein Wnt protein X-Ray Microtomography β-Catenin Belgium United States > US Biomarkers Molecular analysis Bone remodeling Maxillary suture expansion Orthodontics Experimental model
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Summary:	The aim of this study was to analyze the effect of rapid maxillary expansion (RME) on hard tissues. Opening loops bonded to the first and second maxillary molars on both sides were used to apply distracting forces of 0.28 N, 0.42 N and 0.56 N at the midpalatal suture for 7 and 14 days. Microcomputed tomography (MicroCT), histomorphometry and quantitative polymerase chain reaction (qPCR) analysis were performed to evaluate RME effectiveness, midpalatal suture remodeling, cell counting of osteoblasts, osteoclasts and chondrocytes and the expression of bone remodeling markers, respectively. All forces at the two different time points resulted in similar RME and enhanced of bone remodeling. Accordingly, increased number of osteoblasts and reduced chondrocytes counting and no difference in osteoclasts were seen after all RME protocols. RME yielded increased expression of bone remodeling markers as osteocalcin (Ocn), dentin matrix acidic phosphoprotein-1 (Dmp1), runt-related transcription factor 2 (Runx2), collagen type I Alpha 1 (Col1a1), alkaline phosphatase (ALP), receptor activator of nuclear factor kappa B (RANK), receptor activator of nuclear factor kappa B ligand (Rankl), osteoprotegerin (Opg), cathepsin K (Ctsk), matrix metalloproteinases 9 and 13 (Mmp9 and 13), transforming growth fator beta 1, 2 and 3 (Tgfb 1, Tgfb 2 and Tgfb3), bone morphogenetic protein 2 (Bmp-2), sclerostin (Sost), beta-catenin-like protein 1 (Ctnnbl) and Wnt signaling pathways 3, 3a and 5a (Wnt 3, Wnt 3a and Wnt 5a). These findings characterize the cellular changes and potential molecular pathways involved in RME, proving the reliability of this protocol as a model for mechanical-induced bone remodeling.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9290 1873-2380
DOI:	10.1016/j.jbiomech.2020.109880