Search Results - "Rodnina, M. V."

FIDELITY OF AMINOACYL-tRNA SELECTION ON THE RIBOSOME: Kinetic and Structural Mechanisms by Rodnina, Marina V, Wintermeyer, Wolfgang

“…The ribosome discriminates between correct and incorrect aminoacyl-tRNAs (aa-tRNAs), or their complexes with elongation factor Tu (EF-Tu) and GTP, according to…”
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Mechanisms of elongation on the ribosome: dynamics of a macromolecular machine by Wintermeyer, W, Peske, F, Beringer, M, Gromadski, K B, Savelsbergh, A, Rodnina, M V

“…Protein synthesis in the cell is performed on ribosomes, large ribonucleoprotein particles, which in bacteria consist of three RNA molecules and over 50…”
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Visualization of elongation factor Tu on the Escherichia coli ribosome by Heel, Marin van, Stark, Holger, Rodnina, Marina V, Rinke-Appel, Jutta, Brimacombe, Richard, Wintermeyer, Wolfgang

“…The delivery of a specific amino acid to the translating ribosome is fundamental to protein synthesis. The binding of aminoacyl-transfer RNA to the ribosome is…”
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Ten remarks on peptide bond formation on the ribosome by Rodnina, M V, Beringer, M, Bieling, P

“…Peptide-bond formation is the enzymatic activity of the ribosome. The catalytic site is made up of ribosomal RNA, indicating that the ribosome is a ribozyme…”
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EF-G-induced ribosome sliding along the noncoding mRNA by Klimova, M, Senyushkina, T, Samatova, E, Peng, B Z, Pearson, M, Peske, F, Rodnina, M V

“…Translational bypassing is a recoding event during which ribosomes slide over a noncoding region of the messenger RNA (mRNA) to synthesize one protein from two…”
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The Ribosome as an Entropy Trap by Sievers, Annette, Beringer, Malte, Rodnina, Marina V., Wolfenden, Richard

“…To determine the effectiveness of the ribosome as a catalyst, we compared the rate of uncatalyzed peptide bond formation, by the reaction of the ethylene…”
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Kinetic Determinants of High-Fidelity tRNA Discrimination on the Ribosome by Gromadski, Kirill B., Rodnina, Marina V.

“…The ribosome selects aminoacyl-tRNA (aa-tRNA) matching to the mRNA codon from the bulk of nonmatching aa-tRNAs in two consecutive selection steps, initial…”
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GTP hydrolysis by EF-G synchronizes tRNA movement on small and large ribosomal subunits by Holtkamp, Wolf, Cunha, Carlos E, Peske, Frank, Konevega, Andrey L, Wintermeyer, Wolfgang, Rodnina, Marina V

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Ribosomal RNA is the target for oxazolidinones, a novel class of translational inhibitors by MATASSOVA, N.B., RODNINA, M.V., ENDERMANN, R., KROLL, H.-P., PLEISS, U., WILD, H., WINTERMEYER, W.

“…Oxazolidinones are antibacterial agents that act primarily against gram-positive bacteria by inhibiting protein synthesis. The binding of oxazolidinones to 70S…”
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Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A site of the E. coli ribosome by Pape, T, Wintermeyer, W, Rodnina, M V

“…The kinetic mechanism of elongation factor Tu (EF-Tu)-dependent binding of Phe-tRNAPhe to the A site of poly(U)-programmed Escherichia coli ribosomes has been…”
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The ``Allosteric Three-Site Model" of Elongation Cannot Be Confirmed in a Well-Defined Ribosome System from Escherichia coli by Semenkov, Yuri P., Rodnina, Marina V., Wintermeyer, Wolfgang

“…For the functional role of the ribosomal tRNA exit (E) site, two different models have been proposed. It has been suggested that transient E-site binding of…”
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Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome by MATASSOVA, NATALIA B., RODNINA, MARINA V., WINTERMEYER, WOLFGANG

“…During the translocation step of the elongation cycle, two tRNAs together with the mRNA move synchronously and rapidly on the ribosome. The movement is…”
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The G222D mutation in elongation factor Tu inhibits the codon‐induced conformational changes leading to GTPase activation on the ribosome by Vorstenbosch, E., Pape, T., Rodnina, M. V., Kraal, B., Wintermeyer, W.

“…Elongation factor Tu (EF‐Tu) from Escherichia coli carrying the mutation G222D is unable to hydrolyze GTP on the ribosome and to sustain polypeptide synthesis…”
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The ribosome’s response to codon–anticodon mismatches by Daviter, T., Gromadski, K.B., Rodnina, M.V.

“…The ribosome is a molecular machine that synthesizes polypeptides from aminoacyl-tRNAs according to the sequence of the mRNA template. Codon reading by the…”
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Conformational Changes of the Small Ribosomal Subunit During Elongation Factor G-dependent tRNA–mRNA Translocation by Peske, Frank, Savelsbergh, Andreas, Katunin, Vladimir I., Rodnina, Marina V., Wintermeyer, Wolfgang

“…Translocation, a coordinated movement of two tRNAs together with mRNA on the ribosome, is catalyzed by elongation factor G (EF-G). The reaction is accompanied…”
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An Elongation Factor G-Induced Ribosome Rearrangement Precedes tRNA-mRNA Translocation by Savelsbergh, Andreas, Katunin, Vladimir I., Mohr, Dagmar, Peske, Frank, Rodnina, Marina V., Wintermeyer, Wolfgang

“…The elongation cycle of protein synthesis is completed by translocation, a rearrangement during which two tRNAs bound to the mRNA move on the ribosome. The…”
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Truncated Elongation Factor G Lacking the G Domain Promotes Translocation of the 3′End but not of the Anticodon Domain of Peptidyl-tRNA by Borowski, Christian, Rodnina, Marina V., Wintermeyer, Wolfgang

“…The mechanism by which elongation factor G (EF-G) catalyzes the translocation of tRNAs and mRNA on the ribosome is not known. The reaction requires GTP, which…”
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GTPase Activation of Elongation Factors Tu and G on the Ribosome by Mohr, Dagmar, Wintermeyer, Wolfgang, Rodnina, Marina V

“…The GTPase activity of elongation factors Tu and G is stimulated by the ribosome. The factor binding site is located on the 50S ribosomal subunit and comprises…”
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Essential Role of Histidine 84 in Elongation Factor Tu for the Chemical Step of GTP Hydrolysis on the Ribosome by Daviter, Tina, Wieden, Hans-Joachim, Rodnina, Marina V.

“…Elongation factor Tu (EF-Tu) is a GTP-binding protein that delivers aminoacyl-tRNA to the A site of the ribosome during protein synthesis. The mechanism of GTP…”
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Streptomycin interferes with conformational coupling between codon recognition and GTPase activation on the ribosome by Rodnina, Marina V, Gromadski, Kirill B

“…Aminoacyl-tRNAs (aa-tRNAs) are selected by the ribosome through a kinetically controlled induced fit mechanism. Cognate codon recognition induces a…”
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