Purification of xylosaccharides from eucalyptus residues for l‐lactic acid production by Weizmannia coagulans

Purification of xylosaccharides from eucalyptus residues for l‐lactic acid production by Weizmannia coagulans

In this work, phosphoric acid pretreatment (0.6% H3PO4, 160 °C, 40 min) of eucalyptus residues was performed to recover the hemicellulosic fraction for further conversion to l‐lactic acid by fermentation with Weizmannia coagulans DSM 2314 (formerly Bacillus coagulans). The hemicellulosic hydrolysate...

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Published in:Biofuels, bioproducts and biorefining Vol. 18; no. 6; pp. 1902 - 1916
Main Authors: Cabrera, María‐Noel, Vila, Eugenia, Liguori, Alberto, D’Andrada, Constanza, Moure, Santiago, Guigou, Mairan, Cebreiros, Florencia, Rodao, Juan Martín, Camesasca, Laura, Ferrari, Mario Daniel, Lareo, Claudia
Format: Journal Article
Language:English
Published: Chichester, UK John Wiley & Sons, Ltd 01-11-2024
Wiley Subscription Services, Inc
Subjects:
Acetic acid
Acid production
Acids
adsorptive and anionics resin
Amberlite (trademark)
Anion exchange
Anion exchanging
Anions
Consumption
Detoxification
Eucalyptus
Fermentation
Furfural
Hydrolysates
Lactic acid
lignocellulosic residue
Liquor
l‐lactic acid
Performance evaluation
Phosphoric acid
Residues
Resins
Water purification
Weizmannia coagulans
xylosaccharides purification
Xylose
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Summary:In this work, phosphoric acid pretreatment (0.6% H3PO4, 160 °C, 40 min) of eucalyptus residues was performed to recover the hemicellulosic fraction for further conversion to l‐lactic acid by fermentation with Weizmannia coagulans DSM 2314 (formerly Bacillus coagulans). The hemicellulosic hydrolysate was composed of xylosaccharides 24.8 g L–1 (mainly xylose, 20.3 g L–1), acetic acid 7.8 g L–1, furfural 0.7 g L–1, and acid‐soluble lignin (ASL) 2.1 g L–1. It was subsequently purified by using anion exchange or adsorption resins. Different liquor‐to‐resin ratios were evaluated to obtain a high concentration of xylosaccharides in the eluate and thorough removal of components that inhibit lactic fermentation. The best performance was observed when using Amberlite‐XAD‐4 resin at a liquor‐to‐resin ratio of 3:1. An eluted hydrolysate was obtained, preserving 80% of the xylosaccharides and effectively removing almost all furfural, 90% of ASL, and 32% of acetic acid. Subsequently, l‐lactic acid production by W. coagulans DSM 2314 was evaluated using concentrated nondetoxified and detoxified hydrolysate obtained with a vacuum oven. For the nondetoxified hydrolysate, 12 g L–1 of l‐lactic acid was obtained after 48 h showing a yield of 0.56 glactic acid gsugar–1 and a xylose consumption of 62%. The detoxification of the liquor significantly improved the fermentation performance of W. coagulans, resulting in a concentration of 16 g L–1 of lactic acid after 24 h, with a yield of 0.73 glactic acid gsugar–1, and almost complete xylose consumption.
ISSN:1932-104X
1932-1031
DOI:10.1002/bbb.2662