Search Results - "Roco, Charles"

Microbial single-cell RNA sequencing by split-pool barcoding by Kuchina, Anna, Brettner, Leandra M, Paleologu, Luana, Roco, Charles M, Rosenberg, Alexander B, Carignano, Alberto, Kibler, Ryan, Hirano, Matthew, DePaolo, R William, Seelig, Georg

“…Single-cell RNA sequencing (scRNA-seq) has become an essential tool for characterizing gene expression in eukaryotes, but current methods are incompatible with…”
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Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding by Rosenberg, Alexander B, Roco, Charles M, Muscat, Richard A, Kuchina, Anna, Sample, Paul, Yao, Zizhen, Graybuck, Lucas T, Peeler, David J, Mukherjee, Sumit, Chen, Wei, Pun, Suzie H, Sellers, Drew L, Tasic, Bosiljka, Seelig, Georg

“…To facilitate scalable profiling of single cells, we developed split-pool ligation-based transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq…”
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Spatial and cell type transcriptional landscape of human cerebellar development by Aldinger, Kimberly A., Thomson, Zachary, Phelps, Ian G., Haldipur, Parthiv, Deng, Mei, Timms, Andrew E., Hirano, Matthew, Santpere, Gabriel, Roco, Charles, Rosenberg, Alexander B., Lorente-Galdos, Belen, Gulden, Forrest O., O’Day, Diana, Overman, Lynne M., Lisgo, Steven N., Alexandre, Paula, Sestan, Nenad, Doherty, Dan, Dobyns, William B., Seelig, Georg, Glass, Ian A., Millen, Kathleen J.

“…The human neonatal cerebellum is one-fourth of its adult size yet contains the blueprint required to integrate environmental cues with developing motor,…”
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High-throughput single-cell transcriptomics of bacteria using combinatorial barcoding by Gaisser, Karl D., Skloss, Sophie N., Brettner, Leandra M., Paleologu, Luana, Roco, Charles M., Rosenberg, Alexander B., Hirano, Matthew, DePaolo, R. William, Seelig, Georg, Kuchina, Anna

“…Microbial split-pool ligation transcriptomics (microSPLiT) is a high-throughput single-cell RNA sequencing method for bacteria. With four combinatorial…”
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Highly-defective nanocrystals of ZnS formed via dissimilatory bacterial sulfate reduction: A comparative study with their abiogenic analogues by Xu, Jie, Murayama, Mitsuhiro, Roco, Charles M., Veeramani, Harish, Michel, F. Marc, Rimstidt, J. Donald, Winkler, Christopher, Hochella, Michael F.

“…The physicochemical properties of a (nano)mineral are strongly affected by its formation processes, and thus, may indicate the (nano)mineral’s formation…”
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A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes by Grancharova, Tanya, Gerbin, Kaytlyn A., Rosenberg, Alexander B., Roco, Charles M., Arakaki, Joy E., DeLizo, Colette M., Dinh, Stephanie Q., Donovan-Maiye, Rory M., Hirano, Matthew, Nelson, Angelique M., Tang, Joyce, Theriot, Julie A., Yan, Calysta, Menon, Vilas, Palecek, Sean P., Seelig, Georg, Gunawardane, Ruwanthi N.

“…We performed a comprehensive analysis of the transcriptional changes occurring during human induced pluripotent stem cell (hiPSC) differentiation to…”
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Strain-Rate Dependence of Elastic Modulus Reveals Silver Nanoparticle Induced Cytotoxicity by Caporizzo, Matthew Alexander, Roco, Charles M., Ferrer, Maria Carme Coll, Grady, Martha E., Parrish, Emmabeth, Eckmann, David M., Composto, Russell John

“…Force-displacement measurements are taken at different rates with an atomic force microscope to assess the correlation between cell health and cell…”
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Profiling antigen-reactive T cells using high throughput single-cell T cell receptor sequencing by Papalexi, Efthymia, Hariadi, Bryan, Curca, Crina, Roco, Charles, Rosenberg, Alexander

“…Abstract Recent advances in single-cell sequencing allow for simultaneous profiling of T cell receptors (TCRs) and transcriptomes leading to the…”
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Expanding the split-pool combinatorial barcoding toolkit to characterize the effects of aging in mouse TCR diversity by Papalexi, Efthymia, Hariadi, Bryan, Suyama, Alexa, Curca, Crina, Roco, Charles, Rosenberg, Alexander

“…Abstract We recently released a flexible and high throughput single-cell sequencing method that leverages split-pool combinatorial barcoding to simultaneously…”
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Simultaneous profiling of single-cell BCR clonal diversity and whole transcriptomes from diseased and healthy PBMCs in a single experiment using split-pool combinatorial barcoding by Sapre, Ajay, Papalexi, Efthymia, Salvino, Alec, Marrujo, Simone, Gallareta-Olivares, Guillermo, Roco, Charles, Rosenberg, Alexander

“…Abstract Many autoimmune diseases are characterized by aberrant B cells which play a central role in pathogenesis. Each B cell expresses a B cell receptor…”
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High throughput Targeted Transcriptome Sequencing of Bone Marrow Mononuclear Cells identifies a Rare Leukemia-Specific T Cell Subset by Papalexi, Efthymia, Hariadi, Bryan, Kim, Grace, Suyama, Alexa, Roco, Charles, Rosenberg, Alexander

“…Abstract Many applications of scRNA-seq are focused on cell type identification, gene regulatory networks, or biomarker discovery. These applications often do…”
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Targeted transcriptome sequencing enables exponential scaling of combinatorial barcoding in leukemia samples by Kim, Grace, Papalexi, Efthymia, Matulich, Peter, Koehler, Ryan, Schroeder, Sarah, Tran, Vuong, Roco, Charles, Rosenberg, Alexander

“…Abstract Single-cell RNA-sequencing (scRNA-seq) has rapidly spread across multiple research fields, leading to new discoveries. Many applications of scRNA-seq…”
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Improved magnetic regulation of delivery profiles from ferrogels by Kennedy, Stephen, Roco, Charles, Déléris, Alizée, Spoerri, Patrizia, Cezar, Christine, Weaver, James, Vandenburgh, Herman, Mooney, David

“…While providing the ability to magnetically enhance delivery rates, ferrogels have not been able to produce the various types of regulated delivery profiles…”
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Using split-pool combinatorial barcoding to simultaneously characterize the transcriptome and TCR diversity of 1 million T cells by Papalexi, Efthymia, Kim, Grace, Hariadi, Bryan, Schroeder, Sarah, Matulich, Peter, Tran, Vuong, Diaz, Daniel, Roco, Charles, Rosenberg, Alexander

“…Abstract Recent advances in single-cell sequencing allow for simultaneous profiling of T cell receptors (TCRs) and transcriptomes leading to the…”
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Scalable and Highly Sensitive Single Cell RNA-seq through Combinatorial Barcoding by Roco, Charles, O'Connor, Emily, Hills, Ethan, Tran, Vuong, Rosenberg, Alexander

“…Single cell and single nuclei transcriptomic approaches leveraging in situ combinatorial barcoding hold tremendous promise for high throughput applications…”
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Redefining the Etiologic Landscape of Cerebellar Malformations by Aldinger, Kimberly A., Timms, Andrew E., Thomson, Zachary, Mirzaa, Ghayda M., Bennett, James T., Rosenberg, Alexander B., Roco, Charles M., Hirano, Matthew, Abidi, Fatima, Haldipur, Parthiv, Cheng, Chi V., Collins, Sarah, Park, Kaylee, Zeiger, Jordan, Overmann, Lynne M., Alkuraya, Fowzan S., Biesecker, Leslie G., Braddock, Stephen R., Cathey, Sara, Cho, Megan T., Chung, Brian H.Y., Everman, David B., Zarate, Yuri A., Jones, Julie R., Schwartz, Charles E., Goldstein, Amy, Hopkin, Robert J., Krantz, Ian D., Ladda, Roger L., Leppig, Kathleen A., McGillivray, Barbara C., Sell, Susan, Wusik, Katherine, Gleeson, Joseph G., Nickerson, Deborah A., Bamshad, Michael J., Gerrelli, Dianne, Lisgo, Steven N., Seelig, Georg, Ishak, Gisele E., Barkovich, A. James, Curry, Cynthia J., Glass, Ian A., Millen, Kathleen J., Doherty, Dan, Dobyns, William B.

“…Cerebellar malformations are diverse congenital anomalies frequently associated with developmental disability. Although genetic and prenatal non-genetic causes…”
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Abstract 226: Targeted transcriptome sequencing enables exponential scaling of combinatorial barcoding in AML samples by Kim, Grace, Papalexi, Efthymia, Matulich, Peter, Koehler, Ryan, Schroeder, Sarah, Tran, Vuong, Roco, Charles, Rosenberg, Alexander

“…Abstract In the thirteen years since its inception, single-cell RNA-sequencing (scRNA-seq) has rapidly spread across multiple fields of research, leading to…”
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Abstract 248: Using combinatorial barcoding to simultaneously profile the transcriptome and immune repertoire of 1 million T cells by Papalexi, Efthymia, Kim, Grace, Hariadi, Bryan, Schroeder, Sarah, Matulich, Peter, Tran, Vuong, Diaz, Daniel, Roco, Charles, Rosenberg, Alexander

“…Abstract T cells are able to recognize and eliminate a wide variety of immunologic threats while maintaining self-tolerance. Pathogen recognition and clearance…”
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Highly-defective nanocrystals of ZnS formed via dissimilatory bacterial sulfate reduction: A comparative study with their abiogenic analogues by Xu, Jie, Murayama, Mitsuhiro, Roco, Charles M., Veeramani, Harish, Michel, F. Marc, Rimstidt, J. Donald, Winkler, Christopher, Hochella, Michael F.

“…The physicochemical properties of a (nano)mineral are strongly affected by its formation processes, and thus, may indicate the (nano)mineral’s formation…”
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SPLiT-seq reveals cell types and lineages in the developing brain and spinal cord by Rosenberg, Alexander B., Roco, Charles M., Muscat, Richard A., Kuchina, Anna, Sample, Paul, Yao, Zizhen, Gray, Lucas, Peeler, David J., Mukherjee, Sumit, Chen, Wei, Pun, Suzie H., Sellers, Drew L., Tasic, Bosiljka, Seelig, Georg

“…To facilitate scalable profiling of single cells, we developed Split Pool Ligation-based Transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq…”
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