Search Results - "Rocha‐Martin, Javier"

The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support by Abellanas‐Perez, Pedro, Carballares, Diego, Rocha‐Martin, Javier, Fernandez‐Lafuente, Roberto

“…In this article, we have analyzed the interactions between enzyme crowding on a given support and its chemical modification (ethylenediamine modification via…”
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One‐step Synthesis of α‐Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell‐free System by Orrego, Alejandro H., López‐Gallego, Fernando, Espaillat, Akbar, Cava, Felipe, Guisan, José M., Rocha‐Martin, Javier

“…The elevated value of α‐keto acids has pushed scientists to explore more efficient and less expensive alternatives for their synthesis. In this work, an…”
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Oriented immobilization of antibodies onto sensing platforms - A critical review by Gao, Shipeng, Guisán, José M., Rocha-Martin, Javier

“…The immunosensor has been proven a versatile tool to detect various analytes, such as food contaminants, pathogenic bacteria, antibiotics and biomarkers…”
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Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins by Siar, El Hocine, Abellanas-Perez, Pedro, Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…It has been reported that the modification of immobilized glyoxyl-ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of…”
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Adsorption features of reduced aminated supports modified with glutaraldehyde: Understanding the heterofunctional features of these supports by de Andrades, Diandra, Abellanas, Pedro, Carballares, Diego, Alcantara, Andres R., Polizeli, Maria de Lourdes Teixeira de Moraes, Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…Immobilization of enzymes on aminated supports using the glutaraldehyde chemistry may involve three different interactions, cationic, hydrophobic, and covalent…”
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Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts by Guimarães, José R., Carballares, Diego, Tardioli, Paulo W., Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…Four commercial immobilized lipases biocatalysts have been submitted to modifications with different metal (zinc, cobalt or copper) phosphates to check the…”
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Tuning Immobilized Enzyme Features by Combining Solid-Phase Physicochemical Modification and Mineralization by Guimarães, José R., Carballares, Diego, Rocha-Martin, Javier, Tardioli, Paulo W., Fernandez-Lafuente, Roberto

“…Lipase B from Candida antarctica (CALB) and lipase from Thermomyces lanuginosus (TLL) were immobilized on octyl agarose. Then, the biocatalysts were chemically…”
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The Stability of Dimeric D-amino Acid Oxidase from Porcine Kidney Strongly Depends on the Buffer Nature and Concentration by Diego Carballares, Javier Rocha-Martin, Roberto Fernandez-Lafuente

“…The first step of the inactivation of the enzyme D-amino acid oxidase (DAAO) from porcine kidney at pH 5 and 7 is the enzyme subunit dissociation, while FAD…”
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The Effects of Buffer Nature on Immobilized Lipase Stability Depend on Enzyme Support Loading by Pedro Abellanas-Perez, Diego Carballares, Javier Rocha-Martin, Roberto Fernandez-Lafuente

“…The lipases from Thermomyces lanuginosus (TLL) and Candida antarctica (B) (CALB) were immobilized on octyl-agarose beads at 1 mg/g (a loading under the…”
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Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process by da Rocha, Thays N, Morellon-Sterlling, Roberto, Rocha-Martin, Javier, Bolivar, Juan M, Gonçalves, Luciana R B, Fernandez-Lafuente, Roberto

“…Penicillin G acylase (PGA) from was immobilized on vinyl sulfone (VS) agarose. The immobilization of the enzyme failed at all pH values using 50 mM of buffer,…”
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Functionalization of Porous Cellulose with Glyoxyl Groups as a Carrier for Enzyme Immobilization and Stabilization by Martins de Oliveira, Sandro, Velasco-Lozano, Susana, Orrego, Alejandro H, Rocha-Martín, Javier, Moreno-Pérez, Sonia, Fraile, José M, López-Gallego, Fernando, Guisán, Jose Manuel

“…The functionalization of the internal surface of macroporous carriers with glyoxyl groups has proven to highly stabilize a large variety of enzymes through…”
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Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their Applications in Bio-Redox Reactions with In Situ Recycling of Soluble Cofactors by Rocha-Martín, Javier, Rivas, Blanca de las, Muñoz, Rosario, Guisán, José M, López-Gallego, Fernando

“…In bio‐redox cascade reactions that are immobilized on porous supports, mass‐transfer limitations may impede the effective concentration of the cofactor around…”
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Stabilization of Immobilized Lipases by Intense Intramolecular Cross-Linking of Their Surfaces by Using Aldehyde-Dextran Polymers by Orrego, Alejandro H, Ghobadi, Rohollah, Moreno-Perez, Sonia, Mendoza, Adriana Jaime, Fernandez-Lorente, Gloria, Guisan, Jose M, Rocha-Martin, Javier

“…Immobilized enzymes have a very large region that is not in contact with the support surface and this region could be the target of new stabilization…”
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Emerging strategies and integrated systems microbiology technologies for biodiscovery of marine bioactive compounds by Rocha-Martin, Javier, Harrington, Catriona, Dobson, Alan D W, O'Gara, Fergal

“…Marine microorganisms continue to be a source of structurally and biologically novel compounds with potential use in the biotechnology industry. The unique…”
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Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium by Abreu Silveira, Erick, Moreno-Perez, Sonia, Basso, Alessandra, Serban, Simona, Pestana Mamede, Rita, Tardioli, Paulo W, Sanchez Farinas, Cristiane, Rocha-Martin, Javier, Fernandez-Lorente, Gloria, Guisan, Jose M

“…Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the…”
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Mineralization of Lipase from Thermomyces lanuginosus Immobilized on Methacrylate Beads Bearing Octadecyl Groups to Improve Enzyme Features by José R. Guimarães, Diego Carballares, Javier Rocha-Martin, Paulo W. Tardioli, Roberto Fernandez-Lafuente

“…Lipase from Thermomyces lanuginosus (TLL) has been immobilized on Purolite Lifetech® ECR8806F (viz. methacrylate macroporous resin containing octadecyl groups,…”
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Heterofunctional Methacrylate Beads Bearing Octadecyl and Vinyl Sulfone Groups: Tricks to Obtain an Interfacially Activated Lipase from Thermomyces lanuginosus and Covalently Attached to the Support by José R. Guimarães, Diego Carballares, Javier Rocha-Martin, Andrés R. Alcántara, Paulo W. Tardioli, Roberto Fernandez-Lafuente

“…Lipase from Thermomyces lanuginosus (TLL) has been immobilized on a methacrylate macroporous resin coated with octadecyl groups (Purolite Lifetech®® ECR8806F)…”
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Editorial: Designing Carrier-Free Immobilized Enzymes for Biocatalysis by Velasco-Lozano, Susana, Rocha-Martin, Javier, Santos, José C. S. dos

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Tuning Almond Lipase Features by Using Different Immobilization Supports by Oumaima Cherni, Diego Carballares, El Hocine Siar, Pedro Abellanas-Perez, Diandra de Andrades, Javier Rocha-Martin, Sellema Bahri, Roberto Fernandez-Lafuente

“…The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate…”
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Stabilization of Enzymes by Multipoint Covalent Attachment on Aldehyde-Supports: 2-Picoline Borane as an Alternative Reducing Agent by Alejandro H. Orrego, Maria Romero-Fernández, María del Carmen Millán-Linares, María del Mar Yust, José M. Guisán, Javier Rocha-Martin

“…Enzyme immobilization by multipoint covalent attachment on supports activated with aliphatic aldehyde groups (e.g., glyoxyl agarose) has proven to be an…”
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