Ketogenic diet-fed rats have increased fat mass and phosphoenolpyruvate carboxykinase activity

The ketogenic diet (KD), characterized by high fat and low carbohydrate and protein contents, has been proposed to be beneficial in children with epilepsy disorders not helped by conventional anti-epileptic drug treatment. Weight loss and inadequate growth is an important drawback of this diet and m...

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Published in:	Molecular nutrition & food research Vol. 52; no. 11; pp. 1365 - 1371
Main Authors:	Ribeiro, Letícia C, Chittó, Ana L, Müller, Alexandre P, Rocha, Juliana K, Castro da Silva, Mariane, Quincozes-Santos, André, Nardin, Patrícia, Rotta, Liane N, Ziegler, Denize R, Gonçalves, Carlos-Alberto, Da Silva, Roselis S.M, Perry, Marcos L.S, Gottfried, Carmem
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 01-11-2008 WILEY-VCH Verlag WILEY‐VCH Verlag Wiley-VCH-Verl
Subjects:	Adipose Tissue - anatomy & histology Adipose Tissue - drug effects Animals Biological and medical sciences Child Cholesterol - blood Diet, Ketogenic - utilization Epilepsy Epilepsy - prevention & control Feeding. Feeding behavior Food industries Fundamental and applied biological sciences. Psychology Glucose tolerance Humans Ketogenic diet Lipidemia Male PEPCK Phosphoenolpyruvate Carboxykinase (ATP) - metabolism Rats Rats, Wistar Triglycerides - blood Vertebrates: anatomy and physiology, studies on body, several organs or systems Weight Loss - drug effects Weight Loss - physiology Rat PEPCK Epilepsy Rodentia Tolerance Glucose Fat mass Lipidemia Ketogenic diet Glucose tolerance Vertebrata Mammalia Diet Carbohydrate
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Summary:	The ketogenic diet (KD), characterized by high fat and low carbohydrate and protein contents, has been proposed to be beneficial in children with epilepsy disorders not helped by conventional anti-epileptic drug treatment. Weight loss and inadequate growth is an important drawback of this diet and metabolic causes are not well characterized. The aim of this study was to examine body weight variation during KD feeding for 6 wk of Wistar rats; fat mass and adipocyte cytosolic phosphoenolpyruvate carboxykinase (PEPCK) activity were also observed. PEPCK activity was determined based on the [H¹⁴CO₃ ⁻]-oxaloacetate exchange reaction. KD-fed rats gained weight at a less rapid rate than normal-fed rats, but with a significant increment in fat mass. The fat mass/body weight ratio already differed between ketogenic and control rats after the first week of treatment, and was 2.4xhigher in ketogenic rats. The visceral lipogenesis was supported by an increment in adipocyte PEPCK, aiming to provide glycerol 3-phosphate to triacylglycerol synthesis and this fat accumulation was accompanied by glucose intolerance. These data contribute to our understanding of the metabolic effects of the KD in adipose tissue and liver and suggest some potential risks of this diet, particularly visceral fat accumulation.
Bibliography:	http://dx.doi.org/10.1002/mnfr.200700415 ark:/67375/WNG-WFJDLK9S-N Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) FINEP/Rede - No. IBN 01.06.0842-00 ArticleID:MNFR200700415 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) istex:9809C73694A487DCA64EDAC1F55A24E1B9AAAB9A Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1613-4125 1613-4133
DOI:	10.1002/mnfr.200700415