Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood

Analytical and clinical performance of molecular assay used by the Brazilian public laboratory network to detect and discriminate Zika, Dengue and Chikungunya viruses in blood

In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are chal...

Full description

Saved in:
Bibliographic Details
Published in:The Brazilian journal of infectious diseases Vol. 25; no. 2; p. 101542
Main Authors: Ribeiro, Marisa O., Godoy, Daniela T., Fontana-Maurell, Marcela, Costa, Elaine M., Andrade, Elisabete F., Rocha, Daniele R., Ferreira, Antônio G.P., Brindeiro, Rodrigo, Tanuri, Amilcar, Alvarez, Patrícia
Format: Journal Article
Language:English
Published: Brazil Elsevier España, S.L.U 01-03-2021
Contexto
Elsevier
Brazilian Society of Infectious Diseases
Subjects:
Arboviruses
Biochemical assays
Blood
Brazil
Chikungunya
Chikungunya fever
Chikungunya Fever - diagnosis
Chikungunya virus - genetics
Dengue
Dengue - diagnosis
Dengue Virus - genetics
Diagnosis
Humans
INFECTIOUS DISEASES
Laboratories
Medical examination
Molecular detection
Molecular diagnostic techniques
Original
Public health
Real time RT-PCR
Zika
Zika Virus - genetics
Zika Virus Infection - diagnosis
Brazil
Molecular detection
Real time RT-PCR
Dengue
Chikungunya
Arboviruses
Public health
Zika
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are challenges to correctly diagnose these viruses. The simultaneous detection of ZIKV, DENV and CHIKV is an important tool for diagnosis and surveillance. Here, we present the analytical and clinical performance evaluation of ZDC molecular assay (Bio-Manguinhos) at the public health laboratories three years after its registration at ANVISA. The clinical performance demonstrates the ZDC molecular assay (Bio-Manguinhos) has 100% sensitivity and 100% specificity to detect and discriminate ZIKV, CHIKV, and DENV from clinical plasma samples. The ZDC molecular assay (Bio-Manguinhos) results were highly reproducible and no cross-reactivity was seen during testing with a panel of other infectious agents. In conclusion, the ZDC molecular assay (Bio-Manguinhos) is an accurate and reliable tool to monitor Zika, dengue and chikungunya infections in countries like Brazil with simultaneous circulation of the three viruses.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1413-8670
1678-4391
1678-4391
DOI:10.1016/j.bjid.2021.101542