Controlling burst effect with PLA/PVA coaxial electrospun scaffolds loaded with BMP-2 for bone guided regeneration

Biocompatible scaffolds have been used to promote cellular growth and proliferation in order to develop grafts, prostheses, artificial skins and cartilage. Electrospinning is widely studied as a method capable of producing nanofibers which enables cell attachment and proliferation, generating a func...

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Published in:	Materials Science & Engineering C Vol. 97; pp. 602 - 612
Main Authors:	da Silva, Talita Nascimento, Gonçalves, Raquel Pires, Rocha, Carol L., Archanjo, Bráulio S., Barboza, Carlos Augusto G., Pierre, Maria Bernadete R., Reynaud, Franceline, de Souza Picciani, Paulo Henrique
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-04-2019 Elsevier BV
Subjects:	Alcohols Alveolar bone Animals Biocompatibility Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biomaterials Bone and Bones - physiology Bone growth Bone morphogenetic protein 2 Bone Morphogenetic Protein 2 - chemistry Bone Morphogenetic Protein 2 - pharmacology Bone surgery Cartilage Cell adhesion Cell Differentiation - drug effects Cell Line Cell Proliferation - drug effects Cell Survival - drug effects Cellular structure Contact angle Core-shell fiber Crystal structure Drug Carriers - chemistry Electrospinning Fibers Grafts Humans Hydrophobicity Materials science Mice Microscopy Model testing Morphology Nanofibers Nanofibers - chemistry Nanofibers - toxicity Organs Periodontics Polyesters - chemistry Polylactic acid Polymers Polyvinyl Alcohol - chemistry Process parameters Prostheses Prosthetics Proteins Recombinant Proteins - chemistry Recombinant Proteins - pharmacology Regeneration Regeneration - drug effects Scaffolds Skin Thermodynamic properties Thickness Tissue Engineering Tissues Transforming Growth Factor beta - chemistry Transforming Growth Factor beta - pharmacology Ultrasonic testing X-ray diffraction Electrospinning Core-shell fiber Tissue engineering Biomaterials
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Summary:	Biocompatible scaffolds have been used to promote cellular growth and proliferation in order to develop grafts, prostheses, artificial skins and cartilage. Electrospinning is widely studied as a method capable of producing nanofibers which enables cell attachment and proliferation, generating a functional scaffold that is suitable for many types of organs or tissues. In this study, electrospinning was used to obtain core-shell and monolithic fibers from the biocompatible poly (lactic acid) and poly (vinyl alcohol) polymers. The main purpose of this work is to produce core-shell nanofiber based scaffolds that works as a sustained delivery vehicle for BMP-2 protein, allowing those fibers to be used in the recovery of alveolar bone tissue without further bone surgery. Then, polymer nanofibers were manufactured by optimizing process parameters of coaxial electrospinning with emphasis on the most relevant ones: voltage, internal and external flows in an attempt to correlate fibers properties with protein releasing abilities. All nanofibers were characterized according to its morphology, thermal behaviour, crystallinity and release profile. For the release tests, bovine albumin was added into internal fiber for future periodontal restorage application. Obtained results demonstrate that fibers were formed with diameters up to 250 nm. According to electronic microscopy images, one could observe surface of nanofibers, thickness and core-shell morphology confirmed. X-ray diffraction analysis and contact angle tests showed fibers with low crystal degree and low hydrophobicity. Nanofibers structure affected in vitro release model tests and consequently the cellular assays. •This manuscript presents the production of coaxial PLA/PVA electrospun nanofiber scaffolds as a releasing protein model suitable for differentiation of MC3T3-E1 cells.•Here, we demonstrate that main electrospinning process parameters can define microstructure of solidified polymer scaffolds which are responsible in defining the release of encapsuled human bone morphogentetic protein (BMP-2).•Two different release models could be fitted for samples with different structures, mostly governed by the structure of PLA shell.•Moreover, a straight correlation among polymer structure and pproperties and in vitro cell tests could be established.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0928-4931 1873-0191
DOI:	10.1016/j.msec.2018.12.020