Search Results - "Robison, Timothy W."

Erythrocyte PIG‐A mutant frequencies in cancer patients receiving cisplatin by Dobrovolsky, Vasily N., Atiq, Omar T., Heflich, Robert H., Maisha, Mackean, McKinzie, Page B., Pearce, Mason G., Robison, Timothy W.

“…Background Cisplatin is a primary chemotherapy choice for various solid tumors. DNA damage caused by cisplatin results in apoptosis of tumor cells…”
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Monitoring humans for somatic mutation in the endogenous pig-a gene using red blood cells by Dobrovolsky, Vasily N., Elespuru, Rosalie K., Bigger, C. Anita H., Robison, Timothy W., Heflich, Robert H.

“…The endogenous X‐linked PIG‐A gene is involved in the synthesis of glycosyl phosphatidyl inositol (GPI) anchors that tether specific protein markers to the…”
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Current and Future Application of Genetic Toxicity Assays: The Role and Value of In Vitro Mammalian Assays by Elespuru, Rosalie K., Agarwal, Rajiv, Atrakchi, Aisar H., Bigger, C. Anita H., Heflich, Robert H., Jagannath, Devaraya R., Levy, Dan D., Moore, Martha M., Ouyang, Yanli, Robison, Timothy W., Sotomayor, Rene E., Cimino, Michael C., Dearfield, Kerry L.

“…With the advent of new technologies (e.g., genomics, automated analyses, and in vivo monitoring), new regulations (e.g., the reduction of animal tests by the…”
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Appropriate in vivo follow-up assays to an in vitro bacterial reverse mutation (Ames) test positive investigational drug candidate (active pharmaceutical ingredient), drug-related metabolite, or drug-related impurity by Robison, Timothy W., Heflich, Robert H., Manjanatha, Mugimane G., Elespuru, Rosalie, Atrakchi, Aisar, Mei, Nan, Ding, Wei

“…Kirkland et al. [Mutation Research/Genetic Toxicology and Environmental Mutagenesis 847 (2019) 403035, https://doi.org/10.1016/j.mrgentox.2019.03.008; Mutation…”
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Evaluation of mutagenic susceptibility of different stages in germ cell development of Caenorhabditis elegans using whole genome sequencing by Du, Hua, Pan, Bohu, Alund, Alexander W., Yan, Jian, Chen, Ying, Robison, Timothy W., Chen, Tao

“…In contrast to somatic mutations, mutations in germ cells affect every cell of any organism derived from the germ cell and therefore are related to numerous…”
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Performance of HepaRG and HepG2 cells in the high-throughput micronucleus assay for in vitro genotoxicity assessment by Guo, Xiaoqing, Seo, Ji-Eun, Petibone, Dayton, Tryndyak, Volodymyr, Lee, Un Jung, Zhou, Tong, Robison, Timothy W., Mei, Nan

“…The micronucleus (MN) assay is a core test used to evaluate genotoxic potential of xenobiotics. The traditional in vitro MN assay is usually conducted in cells…”
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Quantitative comparison of in vitro genotoxicity between metabolically competent HepaRG cells and HepG2 cells using the high-throughput high-content CometChip assay by Seo, Ji-Eun, Tryndyak, Volodymyr, Wu, Qiangen, Dreval, Kostiantyn, Pogribny, Igor, Bryant, Matthew, Zhou, Tong, Robison, Timothy W., Mei, Nan, Guo, Xiaoqing

“…In vitro genotoxicity testing that employs metabolically active human cells may be better suited for evaluating human in vivo genotoxicity than current…”
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Repeat treatment of organotypic airway cultures with ethyl methanesulfonate causes accumulation of somatic cell mutations without expansion of bronchial-carcinoma-specific cancer driver mutations by Wang, Yiying, Le, Yuan, Harris, Kelly L., Chen, Ying, Li, Xilin, Faske, Jennifer, Wynne, Rebecca A., Mittelstaedt, Roberta A., Cao, Xuefei, Miranda-Colon, Jaime, Elkins, Lana, Muskhelishvili, Levan, Davis, Kelly, Mei, Nan, Sun, Wei, Robison, Timothy W., Heflich, Robert H., Parsons, Barbara L.

“…The human in vitro organotypic air-liquid-interface (ALI) airway tissue model is structurally and functionally similar to the human large airway epithelium…”
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Mutational signatures in T‐lymphocytes of rats treated with N‐propyl‐N‐nitrosourea and procarbazine by Revollo, Javier R., McKinzie, Page B., Robison, Timothy W., Dobrovolsky, Vasily N.

“…We have used whole genome sequencing (WGS) to determine mutational signatures induced in the T‐cells of rats treated in vivo with N‐propyl‐N‐nitrosourea (PNU)…”
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Performance of high-throughput CometChip assay using primary human hepatocytes: a comparison of DNA damage responses with in vitro human hepatoma cell lines by Seo, Ji-Eun, Wu, Qiangen, Bryant, Matthew, Ren, Lijun, Shi, Qiang, Robison, Timothy W., Mei, Nan, Manjanatha, Mugimane G., Guo, Xiaoqing

“…Primary human hepatocytes (PHHs) are considered the “gold standard” for evaluating hepatic metabolism and toxicity of xenobiotics. In the present study, we…”
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Genetic toxicity testing using human in vitro organotypic airway cultures: Assessing DNA damage with the CometChip and mutagenesis by Duplex Sequencing by Wang, Yiying, Mittelstaedt, Roberta A., Wynne, Rebecca, Chen, Ying, Cao, Xuefei, Muskhelishvili, Levan, Davis, Kelly, Robison, Timothy W., Sun, Wei, Schmidt, Elizabeth K., Smith, Thomas H., Norgaard, Zachary K., Valentine, Charles C., Yaplee, Jeffry, Williams, Lindsey N., Salk, Jesse J., Heflich, Robert H.

“…The organotypic human air‐liquid‐interface (ALI) airway tissue model has been used as an in vitro cell culture system for evaluating the toxicity of inhaled…”
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CD59‐deficient bone marrow erythroid cells from rats treated with procarbazine and propyl‐nitrosourea have mutations in the Pig‐a gene by Revollo, Javier R., Dad, Azra, Pearce, Mason G., Mittelstaedt, Roberta A., Casildo, Andrea, Lapidus, Rena G., Robison, Timothy W., Dobrovolsky, Vasily N.

“…Procarbazine (PCZ) and N‐propyl‐N‐nitrosourea (PNU) are rodent mutagens and carcinogens. Both induce GPI‐anchored marker‐deficient mutant‐phenotype red blood…”
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Mutagenicity of silver nanoparticles evaluated using whole-genome sequencing in mouse lymphoma cells by Pan, Bohu, Kaldhone, Pravin R., Alund, Alexander W., Du, Hua, Guo, Xiaoqing, Yan, Jian, Chen, Ying, Zhou, Tong, Robison, Timothy W., Chen, Tao

“…The increasing medical and food applications of silver nanoparticles (AgNPs) raise concerns about their safety, including the potential health consequences of…”
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Pig-a mutations in bone marrow erythroblasts of rats treated with 7,12-dimethyl-benz[a]anthracene by Revollo, Javier R., Dad, Azra, Pearce, Mason G., Mittelstaedt, Roberta A., Robison, Timothy W., Dobrovolsky, Vasily N.

“…•Rats treated with DMBA show increased frequencies of CD59-deficient BMEs.•CD59-deficient BMEs from DMBA-treated rats have mutations in the Pig-a gene.•The…”
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Analysis of mutation in the rat Pig‐a assay: I) studies with bone marrow erythroid cells by Revollo, Javier R., Pearce, Mason G., Dad, Azra, Petibone, Dayton M., Robison, Timothy W., Roberts, Daniel, Dobrovolsky, Vasily N.

“…We have established a flow cytometry‐based Pig‐a assay for rat bone marrow erythroid cells (BMEs). The BME Pig‐a assay uses a DNA‐specific stain and two…”
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Voluntary exploratory data submissions to the US FDA and the EMA: experience and impact by Goodsaid, Federico M, Amur, Shashi, Aubrecht, Jiri, Burczynski, Michael E, Carl, Kevin, Catalano, Jennifer, Charlab, Rosane, Close, Sandra, Cornu-Artis, Catherine, Essioux, Laurent, Fornace, Albert J, Hinman, Lois, Hong, Huixiao, Hunt, Ian, Jacobson-Kram, David, Jawaid, Ansar, Laurie, David, Lesko, Lawrence, Li, Heng-Hong, Lindpaintner, Klaus, Mayne, James, Morrow, Peter, Papaluca-Amati, Marisa, Robison, Timothy W, Roth, John, Schuppe-Koistinen, Ina, Shi, Leming, Spleiss, Olivia, Tong, Weida, Truter, Sharada L, Vonderscher, Jacky, Westelinck, Agnes, Zhang, Li, Zineh, Issam

“…Biomarker strategies are increasingly being applied in drug development to address the challenges posed by heterogeneity in the underlying mechanisms of…”
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Metabolites in safety testing by Robison, Timothy W, Jacobs, Abigail

“…Traditionally, only circulating concentrations of parent drug have been measured in the rodent and nonrodent test species used for drug safety assessments and…”
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Differential enhancement of gamma-glutamyl transpeptidase and gamma-glutamylcysteine synthetase by tert-butylhydroquinone in rat lung epithelial L2 cells by Liu, R M, Hu, H, Robison, T W, Forman, H J

“…Sublethal quinone-mediated oxidative stress stimulates increases in the activities and mRNA levels of gamma-glutamyl transpeptidase (GGT) and…”
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Increased gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase activities enhance resistance of rat lung epithelial L2 cells to quinone toxicity by Liu, R M, Hu, H, Robison, T W, Forman, H J

“…Tert-butylhydroquinone (TBHQ) is a monofunctional Phase II enzyme inducer, which produces reactive oxygen species. Incubation with a sublethal concentration of…”
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Development and characterization of rabbit tracheal epithelial cell monolayer models for drug transport studies by MATHIAS, N. R, KWANG-JIN KIM, ROBISON, T. W, LEE, V. H. L

“…The objective of this study was to investigate how culture conditions would affect the morphological, functional, and permeability characteristics of rabbit…”
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