Search Results - "Robertson, Rae M."

Cooperative Rheological State‐Switching of Enzymatically‐Driven Composites of Circular DNA And Dextran by Marfai, Juexin, McGorty, Ryan J., Robertson‐Anderson, Rae M.

“…Polymer topology, which plays a principal role in the rheology of polymeric fluids, and non‐equilibrium materials, which exhibit time‐varying rheological…”
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Rheology of Entangled Solutions of Ring–Linear DNA Blends by Kong, Dejie, Banik, Sourya, San Francisco, Michael J, Lee, Megan, Robertson Anderson, Rae M, Schroeder, Charles M, McKenna, Gregory B

“…Bulk rheology measurements on concentrated monodisperse ring–linear DNA mixtures are reported for the first time. The entanglement behavior of the ring–linear…”
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From Bioreactor to Bulk Rheology: Achieving Scalable Production of Highly Concentrated Circular DNA by Paiva, Wynter A., Alakwe, Somkene D., Marfai, Juexin, Jennison‐Henderson, Madigan V., Achong, Rachel A., Duche, Tinotenda, Weeks, April A., Robertson‐Anderson, Rae M., Oldenhuis, Nathan J.

“…DNA serves as a model system in polymer physics due to its ability to be obtained as a uniform polymer with controllable topology and nonequilibrium behavior…”
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Diffusion of Isolated DNA Molecules: Dependence on Length and Topology by Robertson, Rae M., Laib, Stephan, Smith, Douglas E.

“…The conformation and dynamics of circular polymers is a subject of considerable theoretical and experimental interest. DNA is an important example because it…”
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Optical Tweezers Microrheology: From the Basics to Advanced Techniques and Applications by Robertson-Anderson, Rae M

“…Over the past few decades, microrheology has emerged as a widely used technique to measure the mechanical properties of soft viscoelastic materials. Optical…”
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Crowding Induces Complex Ergodic Diffusion and Dynamic Elongation of Large DNA Molecules by Chapman, Cole D., Gorczyca, Stephanie, Robertson-Anderson, Rae M.

“…Despite the ubiquity of molecular crowding in living cells, the effects of crowding on the dynamics of genome-sized DNA are poorly understood. Here, we track…”
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Strong effects of molecular topology on diffusion of entangled DNA molecules by Robertson, Rae M, Smith, Douglas E

“…When long polymers such as DNA are in a highly concentrated state they may become entangled, leading to restricted self-diffusion. Here, we investigate the…”
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Optical Tweezers Microrheology Maps the Dynamics of Strain-Induced Local Inhomogeneities in Entangled Polymers by Khan, Manas, Regan, Kathryn, Robertson-Anderson, Rae M

“…Optical tweezers microrheology (OTM) offers a powerful approach to probe the nonlinear response of complex soft matter systems, such as networks of entangled…”
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Effect of molecular architecture on ring polymer dynamics in semidilute linear polymer solutions by Zhou, Yuecheng, Hsiao, Kai-Wen, Regan, Kathryn E., Kong, Dejie, McKenna, Gregory B., Robertson-Anderson, Rae M., Schroeder, Charles M.

“…Understanding the dynamics of ring polymers is a particularly challenging yet interesting problem in soft materials. Despite recent progress, a complete…”
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Optical-Tweezers-integrating-Differential-Dynamic-Microscopy maps the spatiotemporal propagation of nonlinear strains in polymer blends and composites by Peddireddy, Karthik R., Clairmont, Ryan, Neill, Philip, McGorty, Ryan, Robertson-Anderson, Rae M.

“…How local stresses propagate through polymeric fluids, and, more generally, how macromolecular dynamics give rise to viscoelasticity are open questions vital…”
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Self-Diffusion of Entangled Linear and Circular DNA Molecules:  Dependence on Length and Concentration by Robertson, Rae M., Smith, Douglas E.

“…Self-diffusion coefficients (D) of DNA molecules of varying length and concentration were measured by tracking the Brownian motion of individual fluorescently…”
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Direct measurement of the intermolecular forces confining a single molecule in an entangled polymer solution by Robertson, Rae M, Smith, Douglas E

“…We use optical tweezers to directly measure the intermolecular forces acting on a single polymer imposed by surrounding entangled polymers (115 kbp DNA, 1…”
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Nonlinear microrheology reveals entanglement-driven molecular-level viscoelasticity of concentrated DNA by Chapman, Cole D, Robertson-Anderson, Rae M

“…We optically drive a trapped microscale probe through entangled DNA at rates up to 100× the disentanglement rate (Wi≈100), then remove the trap and track…”
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Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites by Sheung, Janet Y, Garamella, Jonathan, Kahl, Stella K, Lee, Brian Y, McGorty, Ryan J, Robertson-Anderson, Rae M

“…The cytoskeleton-a composite network of biopolymers, molecular motors, and associated binding proteins-is a paradigmatic example of active matter. Particle…”
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Varying crosslinking motifs drive the mesoscale mechanics of actin-microtubule composites by Ricketts, Shea N., Francis, Madison L., Farhadi, Leila, Rust, Michael J., Das, Moumita, Ross, Jennifer L., Robertson-Anderson, Rae M.

“…The cytoskeleton precisely tunes its mechanics by altering interactions between semiflexible actin filaments, rigid microtubules, and crosslinking proteins. We…”
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Onset of Non-Continuum Effects in Microrheology of Entangled Polymer Solutions by Chapman, Cole D, Lee, Kent, Henze, Dean, Smith, Douglas E, Robertson-Anderson, Rae M

“…Microrheology has emerged as a powerful approach for elucidating mechanical properties of soft materials and complex fluids, especially biomaterials. In this…”
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Synergistic Interactions Between DNA and Actin Trigger Emergent Viscoelastic Behavior by Fitzpatrick, Robert, Michieletto, Davide, Peddireddy, Karthik R, Hauer, Cole, Kyrillos, Carl, Gurmessa, Bekele J, Robertson-Anderson, Rae M

“…Composites of flexible and rigid polymers are ubiquitous in biology and industry alike, yet the physical principles determining their mechanical properties are…”
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When Ends Meet: Circular DNA Stretches Differently in Elongational Flows by Li, Yanfei, Hsiao, Kai-Wen, Brockman, Christopher A, Yates, Daniel Y, Robertson-Anderson, Rae M, Kornfield, Julia A, San Francisco, Michael J, Schroeder, Charles M, McKenna, Gregory B

“…Chain topology has a profound impact on the flow behavior of single macromolecules. For circular polymers, the absence of free ends results in a unique chain…”
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DNA Conformation Dictates Strength and Flocculation in DNA–Microtubule Composites by Peddireddy, Karthik R, Michieletto, Davide, Aguirre, Gina, Garamella, Jonathan, Khanal, Pawan, Robertson-Anderson, Rae M

“…Polymer topology has been shown to play a key role in tuning the dynamics of complex fluids and gels. At the same time, polymer composites, ubiquitous in…”
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Protocol for analyzing DNA dynamics in the presence of crowders and confined within cell-sized droplets by Aporvari, Mehdi Shafiei, McGorty, Ryan, Robertson-Anderson, Rae M.

“…Here, we present a protocol for encapsulating DNA molecules under crowded conditions within cell-sized lipid-coated droplets. We describe steps for preparing a…”
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