Search Results - "Robertson, J. Brian"
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Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis
Published in Nature communications (17-09-2020)“…Yeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share…”
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Author Correction: Eukaryotic cell biology is temporally coordinated to support the energetic demands of protein homeostasis
Published in Nature communications (08-12-2021)Get full text
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A bioluminescent reporter for the halophilic archaeon Haloferax volcanii
Published in Extremophiles : life under extreme conditions (01-09-2020)“…Haloarchaea have evolved to thrive in hypersaline environments. Haloferax volcanii is of particular interest due to its genetic tractability; however, few in…”
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BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset for repeatedly modifying wild-type Scheffersomyces stipitis
Published in mSphere (24-08-2023)“…is a yeast that robustly ferments the 5-carbon sugar xylose, making the yeast a valuable candidate for lignocellulosic ethanol fermentation. However, the…”
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Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells
Published in Methods in molecular biology (Clifton, N.J.) (2016)“…"pHlash" is a novel bioluminescence-based pH sensor for measuring intracellular pH, which is developed based on Bioluminescence Resonance Energy Transfer…”
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pHlash: a new genetically encoded and ratiometric luminescence sensor of intracellular pH
Published in PloS one (14-08-2012)“…We report the development of a genetically encodable and ratiometic pH probe named "pHlash" that utilizes Bioluminescence Resonance Energy Transfer (BRET)…”
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Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast
Published in Proceedings of the National Academy of Sciences - PNAS (18-11-2008)“…The use of luciferase reporters has become a precise, noninvasive, high-throughput method for real-time monitoring of promoter activity in living cells,…”
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Dual-Color Monitoring Overcomes the Limitations of Single Bioluminescent Reporters in Fast-Growing Microbes and Reveals Phase-Dependent Protein Productivity during the Metabolic Rhythms of Saccharomyces cerevisiae
Published in Applied and environmental microbiology (01-09-2015)“…Luciferase is a useful, noninvasive reporter of gene regulation that can be continuously monitored over long periods of time; however, its use is problematic…”
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Comparison of mental-status scales for predicting mortality on the general wards
Published in Journal of hospital medicine (01-10-2015)“…BACKGROUND Altered mental status is a significant predictor of mortality in inpatients. Several scales exist to characterize mental status, including the AVPU…”
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Luminescence as a continuous real-time reporter of promoter activity in yeast undergoing respiratory oscillations or cell division rhythms
Published in Methods in molecular biology (Clifton, N.J.) (2011)“…This chapter describes a method for generating yeast respiratory oscillations in continuous culture and monitoring rhythmic promoter activity of the culture by…”
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A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens
Published in Microscopy and microanalysis (01-02-2014)“…A microcompressor is a precision mechanical device that flattens and immobilizes living cells and small organisms for optical microscopy, allowing enhanced…”
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CMF1–Rb interaction promotes myogenesis in avian skeletal myoblasts
Published in Developmental dynamics (01-05-2008)“…CMF1 protein is expressed in developing striated muscle before the expression of contractile proteins, and depletion of CMF1 in myoblasts results in inability…”
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Periodic Fermentor Yield and Enhanced Product Enrichment from Autonomous Oscillations
Published in Applied biochemistry and biotechnology (01-05-2009)“…Four decades of work have clearly established the existence of autonomous oscillations in budding yeast culture across a range of operational parameters and in…”
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Specific deletion of CMF1 nuclear localization domain causes incomplete cell cycle withdrawal and impaired differentiation in avian skeletal myoblasts
Published in Experimental cell research (01-10-2006)“…CMF1 is a protein expressed in embryonic striated muscle with onset of expression preceding that of contractile proteins. Disruption of CMF1 in myoblasts…”
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Safety and efficacy of defibrillator charging during ongoing chest compressions: A multi-center study
Published in Resuscitation (01-11-2010)“…Abstract Background Pauses in chest compressions during cardiopulmonary resuscitation have been shown to correlate with poor outcomes. In an attempt to…”
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LEK1 protein expression in normal and dysregulated cardiomyocyte mitosis
Published in The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology (01-09-2005)“…A defining characteristic of embryonic cells is their ability to divide rapidly, even in tissues such as cardiac muscle, which cannot divide once fully…”
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Light-emitting diode flashlights as effective and inexpensive light sources for fluorescence microscopy
Published in Journal of microscopy (Oxford) (01-10-2009)“…Light-emitting diodes (LEDs) are becoming more commonly used as light sources for fluorescence microscopy. We describe the adaptation of a commercially…”
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Abstract 19504: Three Common Mental Status Scoring Systems are Predictive of Adverse Events in the Inpatient Setting
Published in Circulation (New York, N.Y.) (20-11-2012)“…Abstract only Introduction: Altered mental status has been shown to be one of the most accurate predictors of adverse events in hospitalized patients. Many…”
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Safety and Efficacy of Defibrillator Charging During Ongoing Chest Compressions: A Multicenter Study
Published in Resuscitation (01-11-2010)Get full text
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