Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function

Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated...

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Published in:	Journal of applied animal welfare science Vol. 27; no. 4; pp. 635 - 651
Main Authors:	Oliveira, Maria Emilia Franco, Arrais, Aline Matos, Vergani, Gabriel Brun, Novita Esteves, Sergio, Schinaider Do Amaral Pereira, Verônica, Garcia, Alexandre Rossetto, Bastos, Rosemary, Roberto Bourg Melo, Marco, Rios Coelho Alves, Bruna, Ferreira Fonseca, Jeferson
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 01-10-2024 Taylor & Francis Ltd
Subjects:	17β-Estradiol Animal welfare Animals Benzoates Cervix Uteri - drug effects Chorionic gonadotropin Chorionic Gonadotropin - administration & dosage Chorionic Gonadotropin - pharmacology Corpus Luteum - drug effects Diestrus - drug effects Embryo transfer Embryo Transfer - veterinary Estradiol - administration & dosage Estradiol - analogs & derivatives Estradiol - pharmacology Feasibility Feasibility Studies Female Gonadotropins non-surgical Oxytocin Oxytocin - administration & dosage Oxytocin - pharmacology pharmacological Pituitary (anterior) Pregnancy Progesterone Progesterone - administration & dosage Progesterone - pharmacology Sex hormones Sheep, Domestic transcervical Transposition Embryo transfer transcervical animal welfare non-surgical pharmacological
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Summary:	Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1088-8705 1532-7604 1532-7604
DOI:	10.1080/10888705.2022.2141576