Crosslink-free collagen from Cichla ocellaris: Structural characterization by FT-IR spectroscopy and densitometric evaluation

Crosslink-free collagen of skin from peacock bass Cichla ocellaris was successfully isolated and had its structural characteristics analyzed mainly by the combination of two techniques: optical densitometry and FT-IR. The optical densitometry coupled to the electrophoretic profile showed two typical...

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Bibliographic Details
Published in:	Journal of molecular structure Vol. 1176; pp. 751 - 758
Main Authors:	Oliveira, Vagne de Melo, Neri, Robson Coelho de Araújo, Monte, Flávia Thuane Duarte do, Roberto, Nathália Albuquerque, Costa, Helane Maria Silva, Assis, Caio Rodrigo Dias, Santos, Juliana Ferreira, Bezerra, Ranilson Souza, Porto, Ana Lúcia Figueiredo
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 15-01-2019
Subjects:	Collagen FT-IR Optical densitometry Peacock bass Structure FT-IR Peacock bass Structure Collagen Optical densitometry
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Summary:	Crosslink-free collagen of skin from peacock bass Cichla ocellaris was successfully isolated and had its structural characteristics analyzed mainly by the combination of two techniques: optical densitometry and FT-IR. The optical densitometry coupled to the electrophoretic profile showed two typical α chains (α1 and α2), lower content of dimmers and trimmers (β and ɣ chain, respectively) than other extraction methods and without formation of high molecular weight crosslinks (HMC and VMC). UV–visible absorption spectrum was 211 nm. FT-IR spectra analysis of the amide A, amide B, amide Ι, amide ΙI and amide ΙΙI bands were 3276, 2930, 1637, 1547 and 1240 cm−1, respectively, and showed maintenance of the triple helical chain stabilized by hydrogen bonds characterizing it as collagen. Moreover, PSC was soluble in the range from 0 to 3% of salt concentrations, as well as in alkaline media (pH 8). Our results indicate that the two main techniques used were able to identify and characterize the type 1 crosslinks-free collagen from C. ocellaris, which may be suitable for applications that requires higher purification degree. [Display omitted] •Crosslink-free collagen (pepsin-soluble, PSC) of skin from Cichla ocellaris was successfully isolated.•Optical densitometry characterization showed high content of monomeric chains and no HMC and VMC crosslinks.•PSC was classified as type I which present the highest biological and commercial value.
ISSN:	0022-2860 1872-8014
DOI:	10.1016/j.molstruc.2018.09.023