Peptidylarginine deiminase (PAD) 6 is essential for oocyte cytoskeletal sheet formation and female fertility

Peptidylarginine deiminase (PAD) 6 is essential for oocyte cytoskeletal sheet formation and female fertility

Peptidylarginine deiminase 6 (PAD6) is an enzyme that is uniquely expressed in male and female germ cells. To study the function of this enzyme in vivo we generated mice deficient for PAD6. Here we show that inactivation of the PAD6 gene in mice leads to female infertility whereas male fertility is...

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Bibliographic Details
Published in:Molecular and cellular endocrinology Vol. 273; no. 1; pp. 25 - 31
Main Authors: Esposito, G., Vitale, A.M., Leijten, F.P.J., Strik, A.M., Koonen-Reemst, A.M.C.B., Yurttas, P., Robben, T.J.A.A., Coonrod, S., Gossen, J.A.
Format: Journal Article
Language:English
Published: Ireland Elsevier Ireland Ltd 15-07-2007
Elsevier
Subjects:
Actin Cytoskeleton - metabolism
Animals
Citrulline - metabolism
Cytoskeletal sheet
Cytoskeleton - enzymology
Cytoskeleton - metabolism
Cytoskeleton - ultrastructure
Embryo, Mammalian - cytology
Estrous Cycle - physiology
Female
Fertility
Fertility - physiology
Gene Targeting
Hydrolases - deficiency
Hydrolases - metabolism
Knockout
Mice
Microtubules - metabolism
Oocyte
Oocytes - cytology
Oocytes - enzymology
Oocytes - ultrastructure
Ovary - cytology
Ovary - growth & development
PAD6
Protein-Arginine Deiminases
Skin - cytology
Cytoskeletal sheet
Fertility
PAD6
Oocyte
Knockout
Life Sciences
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Summary:Peptidylarginine deiminase 6 (PAD6) is an enzyme that is uniquely expressed in male and female germ cells. To study the function of this enzyme in vivo we generated mice deficient for PAD6. Here we show that inactivation of the PAD6 gene in mice leads to female infertility whereas male fertility is not affected. The absence of the PAD6 protein and consequently absence of citrullination activity in oocytes results in dispersal of the cytoskeletal sheets in oocytes, indicating an essential role of these germ cell-specific structures in zygote/embryo development. PAD6 deficient mice do not show any other overt phenotype. Thus, we identify citrullination as a new regulator of fertility.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0303-7207
1872-8057
0303-7207
DOI:10.1016/j.mce.2007.05.005