Physicochemical Properties of TPP-Crosslinked Chitosan Nanoparticles as Potential Antibacterial Agents

Physicochemical Properties of TPP-Crosslinked Chitosan Nanoparticles as Potential Antibacterial Agents

Chitosan (CS) nanoparticles with antibacterial properties have been synthesized by the ionic gelation method using tripolyphosphate (TPP) as a crosslinking agent. Physicochemical properties of the resulting CS-TPP nanoparticles were examined by FTIR spectroscopy, X-ray diffraction, scanning electron...

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Bibliographic Details
Published in:Fibers and polymers Vol. 22; no. 11; pp. 2954 - 2964
Main Authors: Khoerunnisa, Fitri, Nurhayati, Mita, Dara, Fitri, Rizki, Regita, Nasir, Muhamad, Aziz, Hafiz Aji, Hendrawan, Hendrawan, Poh, Ng Eng, Kaewsaneha, Chariya, Opaprakasit, Pakorn
Format: Journal Article
Language:English
Published: Seoul The Korean Fiber Society 01-11-2021
Springer Nature B.V
한국섬유공학회
Subjects:
Amines
Biological materials
Chemistry
Chemistry and Materials Science
Chitosan
Crosslinking
Diffraction patterns
E coli
Fourier transforms
Infrared spectroscopy
Ionic interactions
Nanoparticles
Polydispersity
Polymer Sciences
Scanning electron microscopy
Spectrum analysis
X-ray diffraction
섬유공학
Nanoparticles CS-TPP
Ionic gelation
Antibacterial activity
Chitosan
Tripolyphosphate
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Summary:Chitosan (CS) nanoparticles with antibacterial properties have been synthesized by the ionic gelation method using tripolyphosphate (TPP) as a crosslinking agent. Physicochemical properties of the resulting CS-TPP nanoparticles were examined by FTIR spectroscopy, X-ray diffraction, scanning electron microscopy, energy dispersive X-ray, transmission electron microscopy, and UV-Vis spectroscopy. The antibacterial efficacy of the nanoparticles against S. aureus and E. coli was evaluated by the disc diffusion (DD) method. It was found that the optimum conditions for fabricating the CS-TPP nanoparticles were: pH of 2.85, CS concentration of 1.5 mg/m l , TPP concentration of 0.5 mg/m l , CS:TPP feed ratio of 1:1, mixing speed of 300 rpm, and reaction temperature of 30 ºC. The CS-TPP nanoparticles decreased in average particle size with an increase in the CS concentration. Based on the DLS results, the average size of CS-TPP ranged from 79.2 to 114.2 nm, with a polydispersity index of 0.369-0.398. However, slightly larger sizes were observed (150 to 180 nm), based on the TEM and SEM images. The CS-TPP has more regular structures compared to CS, indicated by the increase of crystallinity based on X-ray diffraction patterns. In addition, FTIR spectra confirmed that interactions in tripolyphosphate were predominantly between CS amines and TPP counterions via ionic interactions, as supported by SEM-EDX. CS-TPP exhibited antibacterial activity. The highest inhibition zone radius was 5.0 and 5.5 mm against E. coli and S. aureus , respectively. The bio-based materials have a high potential for use as value-added antibacterial agents.
ISSN:1229-9197
1875-0052
DOI:10.1007/s12221-021-0397-z