Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia

Surveillance of canine leishmaniasis in Colombia is restricted to the appearance of visceral leishmaniasis cases in humans, and is mainly performed by serological tests. This requires blood sampling by veterinarians or technicians according to Colombian laws. The aim of this study was to evaluate th...

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Bibliographic Details
Published in:	Journal of vector borne diseases Vol. 61; no. 3; pp. 400 - 405
Main Authors:	Mejía-Chimá, Wilmer A, Hernández-Vera, Samuel D, Pérez-Vargas, Oscar Y, Rivero Rodriguez, Matilde E, Paternina Luis, E, Bejarano Eduar, E
Format:	Journal Article
Language:	English
Published:	India Medknow Publications and Media Pvt. Ltd 01-07-2024 Medknow Publications & Media Pvt. Ltd Wolters Kluwer Medknow Publications
Subjects:	Analysis Animals Antibodies, Protozoan - blood Blood colombia Colombia - epidemiology Conjunctiva - parasitology conjunctival swab Cross-Sectional Studies Diagnosis Diagnosis, Noninvasive Diseases Dog Diseases - diagnosis Dog Diseases - epidemiology Dog Diseases - parasitology Dogs Female Health aspects Health surveillance leishmania infantum Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmaniasis Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - epidemiology Leishmaniasis, Visceral - parasitology Leishmaniasis, Visceral - veterinary Male Medical examination Methods Molecular diagnostic techniques Parasitic diseases pcr Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Serologic Tests - methods Serologic Tests - veterinary Serology visceral leishmaniasis Colombia
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Summary:	Surveillance of canine leishmaniasis in Colombia is restricted to the appearance of visceral leishmaniasis cases in humans, and is mainly performed by serological tests. This requires blood sampling by veterinarians or technicians according to Colombian laws. The aim of this study was to evaluate the utility of conjunctival swabs in the molecular detection of Leishmania in dogs from the municipality of Ovejas, Sucre. The present study was cross-sectional and descriptive. The collection source of samples and information was primary. Blood samples and conjunctival swabs from 121 dogs were analysed by PCR-ITS1 to detect Leishmania spp. Positive samples were used to amplify a conserved region of the Leishmania infantum kinetoplast minicircle. Performance of both sample types was calculated by proportion of positive samples of each type and the degree of agreement between them was determined by Cohen's kappa (κ) agreement index. Leishmania infection was detected in 17.4% (21/121) of blood samples and in 16.5% (20/121) of conjunctival swabs. In total, 28.1% (34/121) of the canines were infected, of which 11.8% (4/34) were infected with L. infantum in the conjunctival swabs and 5.9 % (2/34) in the blood samples. The agreement between blood and conjunctiva was medium (κ = 0.207) by PCR-ITS1 amplification. The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0972-9062 0972-9062
DOI:	10.4103/JVBD.JVBD_182_23