Search Results - "Riss, L."
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Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays
Published in Assay and drug development technologies (01-02-2004)“…Here we show the results of comparing cell viability, cytotoxicity, and apoptosis assays for measuring the time- and dose-dependent toxic effects of tamoxifen…”
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Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Published in Analytical biochemistry (15-04-2009)“…A luminescent method to individually measure the chymotrypsin-like, trypsin-like, or caspase-like activities of the proteasome in cultured cells was developed…”
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New Bioluminogenic Substrates for Monoamine Oxidase Assays
Published in Journal of the American Chemical Society (15-03-2006)“…Novel bioluminogenic substrates were designed for probing monoamine oxidase (MAO) activity based on a simple and effective β-elimination strategy. By modifying…”
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Poly (ADP-ribose) polymerase cleavage monitored in situ in apoptotic cells
Published in BioTechniques (01-04-2001)“…During apoptosis, the activation of a family of cysteine proteases, or caspases, results in proteolytic cleavage of numerous substrates. Antibody probes…”
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"Multiplexed viability, cytotoxicity, and caspase activity assays"
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2015)“…Multiplexed assay chemistries provide for multiple measurements of cellular parameters within a single assay well. This experimental practice is not only more…”
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Applying spaced practice in the schools to teach math vocabulary
Published in Psychology in the schools (01-06-2019)“…Spaced practice, or the distribution of practice opportunities across time, is a well‐known and effective practice for improving retention. However, spaced…”
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Treating Cells as Reagents to Design Reproducible Assays
Published in SLAS discovery (01-12-2021)“…The reproducibility of high-throughput cell-based assays is dependent on having a consistent source of cells for each experiment. Developing an understanding…”
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Apoptosis as a biomarker in chemoprevention trials
Published in Urology (Ridgewood, N.J.) (01-04-2001)“…Monitoring apoptosis in histologic samples is becoming increasingly important as more effort is applied to the study of therapeutic modulators of cell death in…”
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A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Published in Analytical biochemistry (15-07-2007)“…A method to simultaneously determine the relative numbers of live and dead cells in culture by introducing a combination of two fluorogenic substrates or a…”
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Calcium alginate beads as a slow-release system for delivering angiogenic molecules in vivo and in vitro
Published in Journal of cellular physiology (01-08-1992)“…A method previously used in this laboratory for entrapment of tumor cells in alginate beads has been extended to provide a slow release delivery system for…”
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In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screening
Published in Current chemical genomics (11-06-2009)“…In vitro cytotoxicity testing has become an integral aspect of drug discovery because it is a convenient, costeffective, and predictive means of characterizing…”
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Cytotoxicity testing: measuring viable cells, dead cells, and detecting mechanism of cell death
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2011)“…Testing the effects of compounds on the viability of cells grown in culture is widely used as a predictor of potential toxic effects in whole animals. Among…”
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The abnormal positions of the small child's foot: the equipment at your disposal
Published in Archives de pédiatrie : organe officiel de la Société française de pédiatrie (01-06-2010)Get full text
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The abnormal positions of the small child's foot: the physiotherapist job
Published in Archives de pédiatrie : organe officiel de la Société française de pédiatrie (01-06-2010)Get full text
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Bioluminescent assays for ADMET
Published in Expert opinion on drug metabolism & toxicology (01-01-2008)“…Bioluminescent assays couple a limiting component of a luciferase-catalyzed photon-emitting reaction to a variable parameter of interest, while holding the…”
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A bioluminescent assay for monoamine oxidase activity
Published in Analytical biochemistry (15-12-2006)“…This article describes a novel two-step homogeneous bioluminescent assay for monoamine oxidase (MAO) that is simple, sensitive, and amenable to high-throughput…”
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Homogeneous, bioluminescent proteasome assays
Published in Methods in molecular biology (Clifton, N.J.) (01-01-2015)“…Protein degradation is mediated predominantly through the ubiquitin-proteasome pathway. The importance of the proteasome in regulating degradation of proteins…”
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Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium
Published in Cancer research (Chicago, Ill.) (15-07-1987)“…Growth of the mouse mammary epithelial cell line designated COMMA-D has been studied in serum-free medium (SFM) formulated with Ham's F12 and Dulbecco's…”
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Characterization of polyclonal antibodies that distinguish acidic and basic fibroblast growth factors by using western immunoblotting and enzyme-linked immunosorbent assays
Published in Journal of cellular physiology (01-02-1989)“…Rabbit polyclonal antibodies were raised against ovalbumin conjugates of purified bovine brain acidic fibroblast growth factor (aFGF) and a synthetic peptide…”
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