In vitro microdialysis sampling of docetaxel

Microdialysis is a technique that allows sampling compounds from the extracellular fluid in different tissues, such as muscle, lung, and brain. However, the feasibility of using this technique with lipopohilic and high molecular weight compounds has been questioned, since these compounds are less li...

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Bibliographic Details
Published in:	Journal of pharmaceutical and biomedical analysis Vol. 36; no. 4; pp. 807 - 813
Main Authors:	Schuck, Virna J.A., Rinas, Irene, Derendorf, Hartmut
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 19-11-2004 Elsevier Science
Subjects:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Docetaxel Fundamental and applied biological sciences. Psychology General pharmacology Lipophilic drugs Medical sciences Microdialysis Microdialysis - methods Pharmacology. Drug treatments Probe calibration Recovery Taxoids - blood Taxoids - chemistry Probe calibration Microdialysis Lipophilic drugs Recovery Docetaxel Antineoplastic agent Lipophilic compound Taxane derivatives Antimitotic In vitro
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Summary:	Microdialysis is a technique that allows sampling compounds from the extracellular fluid in different tissues, such as muscle, lung, and brain. However, the feasibility of using this technique with lipopohilic and high molecular weight compounds has been questioned, since these compounds are less likely to diffuse through the dialysis membrane. Therefore, it was the objective of this study to investigate the feasibility of doing microdialysis of docetaxel by determining its recovery by the microdialysis probe. Three different methods were investigated: extraction efficiency, retrodialysis, and no-net-flux. For the first two methods, three different concentrations were tested: 2.5, 5, and 9 mg/l. The recovery obtained for each concentration was 49.3 ± 6.7 ( n = 4), 44.6 ± 5.4 ( n = 3), and 34.7 ± 2.1 ( n = 4) by extraction efficiency, and 53.4 ± 7.9 ( n = 3), 61.4 ± 7.6 ( n = 3), and 64.2 ± 1.9 ( n = 3) by retrodialysis, respectively. The average recovery obtained by no-net-flux was 68.7 ± 9.6 ( n = 5). Although it has been reported that microdialysis cannot be applied to lipophilic compounds, the results here show the opposite. The high recoveries obtained for docetaxel in all methods applied show that the compound can diffuse through the probe membrane. Overall, docetaxel seems to be very suitable for microdialysis despite its lipophilicity and high molecular weight.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0731-7085 1873-264X
DOI:	10.1016/j.jpba.2004.07.007