Search Results - "Rigler, Rudolf"

A Single-Photon Avalanche Camera for Fluorescence Lifetime Imaging Microscopy and Correlation Spectroscopy by Vitali, Marco, Terenius, Lars, Zappa, Franco, Rigler, Rudolf, Bronzi, Danilo, Krmpot, Aleksandar J., Nikolic, Stanko N., Schmitt, Franz-Josef, Junghans, Cornelia, Tisa, Simone, Friedrich, Thomas, Vukojevic, Vladana

“…Confocal laser scanning microscopy (CLSM) is commonly used to observe molecules of biological relevance in their native environment, the live cell, and study…”
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The Standard Deviation in Fluorescence Correlation Spectroscopy by Wohland, Thorsten, Rigler, Rudolf, Vogel, Horst

“…The standard deviation (SD) in fluorescence correlation spectroscopy (FCS) has been mostly neglected in applications. However, the knowledge of the correct SD…”
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Memory Landscapes of Single-Enzyme Molecules by Edman, Lars, Rigler, Rudolf

“…Immobilized single horseradish peroxidase enzymes were observed by confocal fluorescence spectroscopy during catalysis of the oxidation reaction of the…”
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Quantitative single-molecule imaging by confocal laser scanning microscopy by Vukojević, Vladana, Heidkamp, Marcus, Ming, Yu, Johansson, Björn, Terenius, Lars, Rigler, Rudolf

“…A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. It relies on fluorescence intensity…”
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Quantitative Study of Synthetic Hox Transcription Factor-DNA Interactions in Live Cells by Vukojević, Vladana, Papadopoulos, Dimitrios K., Terenius, Lars, Gehring, Walter J., Rigler, Rudolf

“…Transcription factor-DNA interactions are life sustaining and therefore the subject of intensive research. In spite of vast effort, quantitative in vivo…”
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Single-Molecule Surface-Enhanced Raman and Fluorescence Correlation Spectroscopy of Horseradish Peroxidase by Bjerneld, Erik J, Földes-Papp, Zeno, Käll, Mikael, Rigler, Rudolf

“…We report on the spectroscopy and enzymatic activity of horseradish peroxidase (HRP) coupled to Ag nanoparticles. We show that vibrational spectra of single…”
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Role of C-peptide in human physiology by Wahren, J, Ekberg, K, Johansson, J, Henriksson, M, Pramanik, A, Johansson, B L, Rigler, R, Jörnvall, H

“…The C-peptide of proinsulin is important for the biosynthesis of insulin but has for a long time been considered to be biologically inert. Data now indicate…”
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Conformational Fluctuations in Single DNA Molecules by Wennmalm, Stefan, Edman, Lars, Rigler, Rudolf

“…Measurement of fluorescent lifetimes of dyetagged DNA molecules reveal the existence of different conformations. Conformational fluctuations observed by…”
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Functional Conformational Motions in the Turnover Cycle of Cholesterol Oxidase by Lerch, Hans-Philipp, Rigler, Rudolf, Mikhailov, Alexander S., Ertl, Gerhard

“…Reexamining experimental data of single-molecule fluorescence correlation spectroscopy for cholesterol oxidase, we find that the existing Michaelis-Menten…”
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Function and specificity of synthetic Hox transcription factors in vivo by Papadopoulos, Dimitrios K, Vukojević, Vladana, Adachi, Yoshitsugu, Terenius, Lars, Rigler, Rudolf, Gehring, Walter J

“…Homeotic (Hox) genes encode transcription factors that confer segmental identity along the anteroposterior axis of the embryo. However the molecular mechanisms…”
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Hydrodynamic Flow Profiling in Microchannel Structures by Single Molecule Fluorescence Correlation Spectroscopy by Gösch, Michael, Blom, Hans, Holm, Johan, Heino, Toni, Rigler, Rudolf

“…In this paper we demonstrate high spatial resolution hydrodynamic flow profiling in silicon wafer based microchannels using single molecule fluorescence…”
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Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy by Widengren, Jerker, Mets, Ülo, Rigler, Rudolf

“…GFPs are upon excitation influenced by many different photophysical and photochemical processes effective over a very broad time scale. Much effort has been…”
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Ethanol/naltrexone interactions at the mu-opioid receptor. CLSM/FCS study in live cells by Vukojević, Vladana, Ming, Yu, D'Addario, Claudio, Rigler, Rudolf, Johansson, Björn, Terenius, Lars

“…Alcoholism is a widespread chronic disorder of complex aetiology with a significant negative impact on the individual and the society. Mechanisms of ethanol…”
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Protonation kinetics of GFP and FITC investigated by FCS — aspects of the use of fluorescent indicators for measuring pH by Widengren, Jerker, Terry, Bob, Rigler, Rudolf

“…In this work, the protonation kinetics of fluorescein isothiocyanate (FITC) and a F64L S65T variant (BioST) of green fluorescent protein (GFP) has been…”
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Visualization of a Functionally Enhanced GFP-Tagged Galanin R2 Receptor in PC12 Cells: Constitutive and Ligand-Induced Internalization by Xia, Sheng, Kjær, Svend, Zheng, Kang, Hu, Ping-Sheng, Bai, Li, Jia, Jun-Yong, Rigler, Rudolf, Pramanik, Aladdin, Xu, Tao, Hökfelt, Tomas, Xu, Zhi-Qing David

“…Trafficking of the galanin R2 receptor (GALR2) fused with enhanced GFP (EGFP) was studied by using confocal fluorescence microscopy. The fusion protein was…”
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Fast interactions between Rh6G and dGTP in water studied by fluorescence correlation spectroscopy by Widengren, Jerker, Dapprich, Johannes, Rigler, Rudolf

“…The feasibility of fluorescence correlation spectroscopy (FCS) for the monitoring of fast kinetic processes has been investigated. The interaction between Rh6G…”
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Direct quantification of mRNA expression levels using single molecule detection by Camacho, Agnès, Korn, Kerstin, Damond, Martine, Cajot, Jean-François, Litborn, Erik, Liao, Bohao, Thyberg, Per, Winter, Holger, Honegger, Adrian, Gardellin, Paola, Rigler, Rudolf

“…Determination of the gene expression by direct quantification of mRNA is becoming increasingly important in basic, pharmaceutical and clinical research. We…”
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Triplet-state monitoring by fluorescence correlation spectroscopy by Widengren, J, Rigler, R, Mets, U

“…The effects of high excitation intensities in fluorescence correlation spectroscopy (FCS) in terms of saturation and triplet-state build-up have been studied…”
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Direct gene expression analysis by Winter, Holger, Korn, Kerstin, Rigler, Rudolf

“…The direct analysis of single biological molecules is getting increasingly important in basic as well as pharmaceutical research (e.g. for gene expression…”
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Fluorescence and single molecule analysis in cell biology by Rigler, Rudolf

“…An overview is presented which describes the development of fluorescence spectroscopy at the cellular level from its beginning as a quantitative tool to…”
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