Low‐level laser therapy enhances the number of osteocytes in calvaria bone defects of ovariectomized rats

Low‐level laser therapy enhances the number of osteocytes in calvaria bone defects of ovariectomized rats

Background Osteoporosis can make bone repair difficult. Low‐level laser therapy (LLLT) has been shown to be a promising tool for bone neoformation. This study aimed to analyze the effect of LLLT on calvaria bone defects of ovariectomized rats using stereology. Methods Fifty‐four Wistar rats were sub...

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Published in:Animal models and experimental medicine Vol. 2; no. 1; pp. 51 - 57
Main Authors: Scalize, Priscilla Hakime, Sousa, Luiz Gustavo, Gonçalves, Lígia Maria Napolitano, Pitol, Dimitrius Leonardo, Palinkas, Marcelo, Coppi, Antônio Augusto, Righeti, Mariah Acioli, Ricardo, Vitória, Bombonato‐Prado, Karina Fittipaldi, Regalo, Simone Cecílio Hallak, Siessere, Selma
Format: Journal Article
Language:English
Published: United States John Wiley & Sons, Inc 01-03-2019
John Wiley and Sons Inc
Subjects:
Animals
bone
Bone healing
Bones
Calvaria
Defects
Energy
Fractures
Lasers
low‐level laser therapy
Osteocytes
Osteoporosis
Ovariectomy
Short Communication
Statistical analysis
Surgery
Brazil
bone
osteocytes
osteoporosis
low‐level laser therapy
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Summary:Background Osteoporosis can make bone repair difficult. Low‐level laser therapy (LLLT) has been shown to be a promising tool for bone neoformation. This study aimed to analyze the effect of LLLT on calvaria bone defects of ovariectomized rats using stereology. Methods Fifty‐four Wistar rats were subjected to bilateral ovariectomy, and bone defects were created in calvaria after 150 days. The animals were divided into nine groups (n =  6 per group), and 24 hours after the bone defects were created they received 3, 6 or 12 sessions of LLLT at 0, 20 or 30 J/cm2, using a 780‐nm low‐intensity GaAlAs laser. One‐way ANOVA followed by Tukey's post hoc test was used for data processing. A difference of P < 0.05 was considered statistically significant. The parameters evaluated were osteocyte density (Nvost), total osteocyte number (Ntoost), trabecular surface density (Svt), and trabecular surface area (Sat). Results Data obtained showed that Ntoost, Svt, and Sat in group G2 rats were significantly different from G1 (0 J/cm2) (P < 0.05). Compared to group G4, G5 presented higher values for the parameters Svt and Sat, and G6 presented significantly higher values for almost all the analyzed parameters (Nvost, Ntoost, Svt, and Sat) (P < 0.05). Compared to group G7, G8 showed a higher value only for the parameter Sat, and G9 showed significantly higher values for parameters Nvost, Ntoost, Svt, and Sat. Conclusion We conclude that LLLT stimulated bone neoformation and contributed to an increase in the total number of osteocytes, especially with a laser energy density of 30 J/cm2 given for 6 and 12 sessions.
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ISSN:2576-2095
2096-5451
2576-2095
DOI:10.1002/ame2.12056