Search Results - "Rigaut, G."

A generic protein purification method for protein complex characterization and proteome exploration by Séraphin, Bertrand, Rigaut, Guillaume, Shevchenko, Anna, Rutz, Berthold, Wilm, Matthias, Mann, Matthias

“…We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification…”
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Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition by Fortes, P, Bilbao-Cortés, D, Fornerod, M, Rigaut, G, Raymond, W, Séraphin, B, Mattaj, I W

“…The characterization of a novel yeast-splicing factor, Luc7p, is presented. The LUC7 gene was identified by a mutation that causes lethality in a yeast strain…”
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Statistical and theoretical analysis of precipitates in dual-phase steels microalloyed with titanium and their effect on mechanical properties by Soto, R., Saikaly, W., Bano, X., Issartel, C., Rigaut, G., Charai, A.

“…Transmission electron microscopy was used to study the precipitation in four different dual-phase steels microalloyed with titanium. TiN, Ti 4C 2S 2 and TiC…”
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The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification by Puig, Oscar, Caspary, Friederike, Rigaut, Guillaume, Rutz, Berthold, Bouveret, Emmanuelle, Bragado-Nilsson, Elisabeth, Wilm, Matthias, Séraphin, Bertrand

“…Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to…”
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Sm-like protein complex that participates in mRNA degradation by Bouveret, E, Rigaut, G, Shevchenko, A, Wilm, M, Seraphin, B

“…In eukaryotes, seven Sm proteins bind to the U1, U2, U4 and U5 spliceosomal snRNAs while seven Smlike proteins (Lsm2p–Lsm8p) are associated with U6 snRNA…”
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The S. cerevisiae SET3 complex includes two histone deacetylases, Hos2 and Hst1, and is a meiotic-specific repressor of the sporulation gene program by Pijnappel, W W, Schaft, D, Roguev, A, Shevchenko, A, Tekotte, H, Wilm, M, Rigaut, G, Séraphin, B, Aasland, R, Stewart, A F

“…Set3 is one of two proteins in the yeast Saccharomyces cerevisiae that, like Drosophila Trithorax, contains both SET and PHD domains. We found that Set3 forms…”
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Cotranscriptional spliceosome assembly and splicing are independent of the Prp40p WW domain by Görnemann, Janina, Barrandon, Charlotte, Hujer, Katja, Rutz, Berthold, Rigaut, Guillaume, Kotovic, Kimberly M, Faux, Céline, Neugebauer, Karla M, Séraphin, Bertrand

“…Complex cellular functions involve large networks of interactions. Pre-mRNA splicing and transcription are thought to be coupled by the C-terminal domain (CTD)…”
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Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p by Schmitt, Christel, von Kobbe, Cayetano, Bachi, Angela, Panté, Nelly, Rodrigues, João P., Boscheron, Cécile, Rigaut, Guillaume, Wilm, Matthias, Séraphin, Bertrand, Carmo-Fonseca, Maria, Izaurralde, Elisa

“…Dbp5 is a DEAD‐box protein essential for mRNA export from the nucleus in yeast. Here we report the isolation of a cDNA encoding human Dbp5 (hDbp5) which is 46%…”
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The effects of thermomechanical processing on the precipitation in an industrial dual-phase steel microalloyed with titanium by SAIKALY, W, BANO, X, ISSARTEL, C, RIGAUT, G, CHARRIN, L, CHARAI, A

“…Analytical transmission electron microscopy was employed to characterize the precipitation at each step of the fabrication process and thermomechanical…”
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