Purification and Molecular Analysis of an Extracellular γ-Glutamyl Hydrolase Present in Young Tissues of the Soybean Plant

A polypeptide present in intercellular wash fluids of young leaves ofGlycine maxhas been purified to electrophoretic homogeneity. The protein has been identified as γ-glutamyl hydrolase (GGH) based on the shared homology with a recently cloned cDNA from rat. The enzyme is present within the extracel...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 228; no. 1; pp. 1 - 6
Main Authors:	Huangpu, J., Pak, J.H., Graham, M.C., Rickle, S.A., Graham, J.S.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-11-1996
Subjects:	Blotting, Western Cell Wall - enzymology Cross Reactions DNA, Complementary - genetics FEUILLE gamma-Glutamyl Hydrolase - chemistry gamma-Glutamyl Hydrolase - genetics gamma-Glutamyl Hydrolase - isolation & purification gamma-Glutamyl Hydrolase - metabolism Gene Expression GENETICA MOLECULAR GENETIQUE MOLECULAIRE GLYCINE MAX Glycine max - enzymology HIDROLASAS HOJAS HYDROLASE Metalloendopeptidases - immunology Molecular Sequence Data PARED CELULAR PAROI CELLULAIRE PEPTIDASAS PEPTIDASE PEPTIDE PEPTIDOS Plant Leaves - enzymology PURIFICACION PURIFICATION RACINE RAICES RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant - genetics RNA, Plant - metabolism TALLO TIGE
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Summary:	A polypeptide present in intercellular wash fluids of young leaves ofGlycine maxhas been purified to electrophoretic homogeneity. The protein has been identified as γ-glutamyl hydrolase (GGH) based on the shared homology with a recently cloned cDNA from rat. The enzyme is present within the extracellular space of young leaves and a portion is bound to the cell wall. Northern and Western analysis confirm that this polypeptide is expressed only in young (1-15 d old) leaf, stem and root tissue and is therefore expressed under a strict developmental program. The primary sequence of γ-glutamyl hydrolase shares amino acid identity with a cDNA clone from rat and two partially sequenced cDNAs fromArabidopsis.Although the completein vivofunction of γ-glutamyl hydrolase in plants is unclear, it is known that the protein plays a critical role in folate metabolism and therefore likely in meeting the physiological demands of growing plant tissues.
Bibliography:	9701356 F60 F30 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1996.1608