Microsatellite instability and loss of heterozygosity in radiation-associated thyroid carcinomas of Belarussian children and adults

DNA from 129 paired thyroid tumorous and non-tumorous tissue samples of Belarussian children (102 patients; age at surgery ≤18 years) and adults (27 patients; age at surgery 19–35 years), who had been exposed to radioactive fallout from the Chernobyl reactor accident in 1986, was examined for micros...

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Bibliographic Details
Published in:	Carcinogenesis (New York) Vol. 20; no. 12; pp. 2247 - 2252
Main Authors:	Richter, Hedwig E., Lohrer, Horst D., Hieber, Ludwig, Kellerer, Albrecht M., Lengfelder, Edmund, Bauchinger, Manfred
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-12-1999 Oxford Publishing Limited (England)
Subjects:	adenomatous polyposis coli Adolescent Adult APC avian myelocytomatosis Base Sequence Belarus Biological and medical sciences Biological effects of radiation BRCA1/BRCA2 breast cancer genes 1 and 2 Child DCC deleted colorectal cancer DNA Primers Endocrinopathies feline sarcoma Female FES Fundamental and applied biological sciences. Psychology Genetic Markers hereditary non-polyposis colorectal cancer hMLH1 HNPCC HPRT human MutL-homolog 1 Humans hypoxanthine phosphoribosyltransferase intron V LOH Loss of Heterozygosity Male Malignant tumors Medical sciences microsatellite instability Microsatellite Repeats MSI MYC Neoplasms, Radiation-Induced - genetics Power Plants Radioactive Hazard Release Radiocontamination receptor-typ tyrosine kinase Republic of Belarus RET RETINT5 Thyroid Neoplasms - genetics Thyroid. Thyroid axis (diseases) Tissues, organs and organisms biophysics Ukraine Ukraine, Chernobyl X-ray repair cross complementing 1 and 5 XRCC1 and XRCC5 Belarus Eastern Europe Europe Republic of Belarus Ukraine Endocrinopathy Human Thyroid diseases Radiation Radioactive contamination Thyroid gland Malignant tumor Epidemiology Accident Nuclear power plant Microsatellite DNA Loss of heterozygosity Adult Instability Child
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Summary:	DNA from 129 paired thyroid tumorous and non-tumorous tissue samples of Belarussian children (102 patients; age at surgery ≤18 years) and adults (27 patients; age at surgery 19–35 years), who had been exposed to radioactive fallout from the Chernobyl reactor accident in 1986, was examined for microsatellite instability (MSI) and loss of heterozygosity (LOH). Twenty-eight microsatellite markers were chosen because of their vicinity to DNA repair genes or genes involved in tumorigenesis as well as regions of chromosomal breakpoints in thyroid tumours. In 40 patients (31% of 129) we detected a total of 73 alterations, 80% of which were classified as LOH and only 20% as MSI. Amongst these 40 patients we identified a subgroup of 11, mainly young female patients (8.5% of 129), exhibiting alterations in at least two microsatellite markers. For comparison we examined samples from spontaneous thyroid carcinomas without radiation history from 20 adult patients from Munich (mean age at surgery 56 ± 13 years). None of the tumour samples investigated showed evidence of alterations in the 28 microsatellite markers tested. Taken together our data indicate an increased instability of microsatellite markers in thyroid cancers from Belarussian patients. At present, it is uncertain whether the increased genome instability observed in Belarussian patients is the result of the exposure to radioactive iodine from the Chernobyl reactor accident or due to the young age of the patients.
Bibliography:	PII:1460-2180 ark:/67375/HXZ-5TWB2ZT2-P istex:6C8645B1318679BEBD516A2E8A68BD16E0466BA6 local:0202247 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0143-3334 1460-2180
DOI:	10.1093/carcin/20.12.2247