Search Results - "Ribela, Maria Teresa C.P"
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Human thyroid-stimulating hormone synthesis in human embryonic kidney cells and related N-glycoprofiling analysis for carbohydrate composition determination
Published in Applied microbiology and biotechnology (01-02-2018)“…A strain of embryonic human kidney cells (HEK293) was transiently co-transfected with the expression vectors coding for the α- and β-subunits of human…”
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2
Molecular cloning, sequence analysis and expression of the snake follicle-stimulating hormone receptor
Published in General and comparative endocrinology (01-07-2004)“…Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) control gonadal function in mammalian and many non-mammalian vertebrates through the…”
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Inflammatory oedema induced by phospholipases A2 isolated from Crotalus durissus sp. in the rat dorsal skin: a role for mast cells and sensory C-fibers
Published in Toxicon (Oxford) (01-06-2003)“…The ability of the phospholipases A(2) (PLA(2)s) from Crotalus durissus cascavella, Crotalus durissus collilineatus and Crotalus durissus terrificus venoms and…”
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4
Determination of Chinese hamster ovary cell-derived recombinant thyrotropin by reversed-phase liquid chromatography
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (25-04-2003)“…A reversed-phase high-performance liquid chromatography (RP-HPLC) methodology for the qualitative and quantitative analysis of human thyrotropin (hTSH) in CHO…”
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5
High-level expression of human thyroid-stimulating hormone in Chinese hamster ovary cells by co-transfection of dicistronic expression vectors followed by a dual-marker amplification strategy
Published in Biotechnology and applied biochemistry (01-02-2002)“…The utilization of dicistronic mRNA expression vectors, containing the gene of interest upstream of an amplifiable marker gene, has shown success in rapidly,…”
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High-yield purification of biosynthetic human growth hormone secreted in Escherichia coli periplasmic space
Published in Journal of Chromatography A (13-08-1999)“…A six-step, high-yield purification procedure for the preparation of clinical grade recombinant human growth hormone (rhGH) secreted in bacterial periplasmic…”
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7
Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (01-01-2018)“…•A HPLC method for direct analysis of IFN-α2 in periplasmic extracts.•A HPLC method for an accurate quali- and quantitative analysis of IFN-α2.•A fast method…”
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8
Enhancement of Human Thyrotropin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture
Published in Applied biochemistry and biotechnology (01-12-2013)“…The influence of sodium butyrate (NaBu) on the synthesis of recombinant human thyrotropin (r-hTSH) by CHO cells was investigated for the first time. A…”
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9
N-Glycoprofiling Analysis for Carbohydrate Composition and Site-Occupancy Determination in a Poly-Glycosylated Protein: Human Thyrotropin of Different Origins
Published in International journal of molecular sciences (03-02-2017)“…Human thyrotropin (hTSH) is a glycoprotein with three potential glycosylation sites: two in the α-subunit and one in the β-subunit. These sites are not always…”
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10
Human Macroprolactin Displays Low Biological Activity via Its Homologous Receptor in a New Sensitive Bioassay
Published in The journal of clinical endocrinology and metabolism (01-03-2006)“…Context: Macroprolactinemia is a frequent finding in hyperprolactinemic individuals, usually without clinical impact. Data on biological activity of…”
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11
Stable expression of a human-like sialylated recombinant thyrotropin in a Chinese hamster ovary cell line expressing α2,6-sialyltransferase
Published in Protein expression and purification (01-09-2009)“…A CHO cell line, previously genetically modified by the introduction of rat α2,6-sialyltransferase cDNA, generated for the first time a human-like sialylated…”
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12
Inflammatory oedema induced by Lachesis muta muta (Surucucu) venom and LmTX-I in the rat paw and dorsal skin
Published in Toxicon (Oxford) (2009)“…The ability of crude venom and a basic phospholipase A 2 (LmTX-I) from Lachesis muta muta venom to increase the microvascular permeability in rat paw and skin…”
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13
Role of substance P and bradykinin in acute pancreatitis induced by secretory phospholipase A2
Published in Pancreas (01-07-2008)“…Secretory phospholipases A2 (sPLA2s) induce acute pancreatitis when injected into the common bile duct of rats. Substance P via neurokinin 1 (NK-1) receptors…”
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14
Analysis of intact human follicle-stimulating hormone preparations by reversed-phase high-performance liquid chromatography
Published in Journal of Chromatography A (08-12-2006)“…A reversed-phase high-performance liquid chromatography (RP-HPLC) method for the qualitative and quantitative analysis of intact human follicle-stimulating…”
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15
Expression, purification, and characterization of authentic mouse prolactin obtained in Escherichia coli periplasmic space
Published in Biotechnology and applied biochemistry (01-05-2012)“…Prolactin (PRL) is a pleiotropic hormone produced by lactotroph cells of the anterior pituitary gland and is mainly related to lactation control and…”
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Characterization of the mechanisms underlying the inflammatory response to Polistes lanio lanio (paper wasp) venom in mouse dorsal skin
Published in Toxicon (Oxford) (2009)“…Stings by Polistes wasps can cause life-threatening allergic reactions, pain and inflammation. We examined the changes in microvascular permeability and…”
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Influence of a Reduced CO2 Environment on the Secretion Yield, Potency and N-Glycan Structures of Recombinant Thyrotropin from CHO Cells
Published in Molecular biotechnology (01-06-2008)“…A consistent increase of ∼60% in the secretion yield of CHO-derived hTSH was observed by changing cell culture CO 2 conditions from 5% CO 2 to an air…”
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Two-step chromatographic purification of recombinant human thyrotrophin and its immunological, biological, physico-chemical and mass spectral characterization
Published in Journal of Chromatography A (07-01-2005)“…A purification strategy for rapidly obtaining recombinant human thyrotropin (rhTSH) was designed based on size exclusion and reversed-phase high-performance…”
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19
Characterization of the acute pancreatitis induced by secretory phospholipases A2 in rats
Published in Toxicon (Oxford) (15-12-2005)“…Acute pancreatitis (AP) is an inflammatory disease of the pancreas characterized by local inflammation and extrapancreatic effects such as lung injury…”
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20
Characterization of the acute pancreatitis induced by secretory phospholipases A 2 in rats
Published in Toxicon (Oxford) (2005)“…Acute pancreatitis (AP) is an inflammatory disease of the pancreas characterized by local inflammation and extrapancreatic effects such as lung injury…”
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