Potential inhibitory effect of LASSBio-596, a new thalidomide hybrid, on inflammatory corneal angiogenesis in rabbits

Evaluate the effect of LASSBio-596, structurally designed as a new hybrid of thalidomide, on inflammatory corneal angiogenesis. Eighteen rabbits were submitted to an alkaline cauterization in the right cornea. The animals were randomly allocated to three groups: vehicle, dexamethasone and LASSBio-59...

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Bibliographic Details
Published in:	Ophthalmic research Vol. 48; no. 4; p. 177
Main Authors:	Ribeiro, Joao Crispim M L, Vagnaldo Fechine, Francisco, Ribeiro, Mariana Zaira M L, Barreiro, Eliezer J, Lima, Lidia Moreira, Ricardo, Nagila M P S, Amaral de Moraes, Maria Elisabete, Odorico de Moraes, Manoel
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-2012
Subjects:	Animals Corneal Neovascularization - diagnosis Corneal Neovascularization - drug therapy Dexamethasone - pharmacology Disease Models, Animal Glucocorticoids - pharmacology Keratitis - diagnosis Keratitis - drug therapy Male Ophthalmic Solutions Phosphodiesterase Inhibitors - administration & dosage Phosphodiesterase Inhibitors - chemistry Phosphodiesterase Inhibitors - therapeutic use Phthalic Acids Phthalimides - administration & dosage Phthalimides - chemistry Phthalimides - therapeutic use Rabbits Sulfonamides Thalidomide - chemistry
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Summary:	Evaluate the effect of LASSBio-596, structurally designed as a new hybrid of thalidomide, on inflammatory corneal angiogenesis. Eighteen rabbits were submitted to an alkaline cauterization in the right cornea. The animals were randomly allocated to three groups: vehicle, dexamethasone and LASSBio-596. Drugs were administered by eyedrops 3 times a day for 21 days. Evaluations were performed on days 3, 6, 9, 12, 15, 18 and 21 after cauterization. At these time points, digital images of the cornea were captured in a standard fashion. The angiogenic response was measured using software that was developed specifically for this purpose. It calculated the following parameters: neovascularization area (NA), total vascular length (TVL) and blood vessel number (BVN). It was observed that dexamethasone significantly decreased NA, TVL and BVN during all assessments. From the NA the angiogenesis rate (AR) was calculated in each group. Therefore, dexamethasone completely inhibited the inflammatory corneal angiogenesis with an AR of -0.001 ± 0.006 mm(2)/day, which was significantly lower (p < 0.001) than that observed after treatment with vehicle (0.078 ± 0.024 mm(2)/day) and LASSBio-596 (0.054 ± 0.012 mm(2)/day). Although LASSBio-596 reduced angiogenesis in relation to vehicle, according to NA, TVL and BVN values, this difference was not statistically significant. However, it was found that the AR as measured in the LASSBio-596 group was significantly lower (p < 0.05) than that seen in control animals, indicating a potential antiangiogenic effect. We conclude that topical application of LASSBio-596 at 1.0% has a potential inhibitory effect on inflammatory corneal angiogenesis in rabbits.
ISSN:	1423-0259
DOI:	10.1159/000337137