Melatonin alleviates arsenic‐induced liver injury by regulating protein RKIP and enhancing antioxidant defencse mechanisms

Arsenic (As) is a highly toxic metal and one of the main factors in cancer development through oxidative stress and production of reactive oxygen species. Prior research has demonstrated melatonin's potential as a free radical scavenger. Raf kinase inhibitory protein (RKIP) is an important regu...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of biochemical and molecular toxicology Vol. 38; no. 9; pp. e23835 - n/a
Main Authors:	Rezvankhah, Soheil, Zargari, Felor, Sharifi, Rasoul
Format:	Journal Article
Language:	English
Published:	United States Wiley Subscription Services, Inc 01-09-2024
Subjects:	Animal tissues Animals antioxidant enzymes Antioxidants Antioxidants - metabolism Antioxidants - pharmacology Arsenic Arsenic - toxicity Arsenite Arsenites - toxicity Cancer Chemical and Drug Induced Liver Injury - drug therapy Chemical and Drug Induced Liver Injury - metabolism Chemical and Drug Induced Liver Injury - prevention & control Enzymatic activity Enzyme activity Enzymes Free radicals Hepatocytes Intracellular signalling Kinases Liver Liver - drug effects Liver - metabolism Liver cancer Male Malondialdehyde - metabolism Melatonin Melatonin - pharmacology Oxidative stress Oxidative Stress - drug effects Phosphatidylethanolamine Binding Protein - metabolism Protein expression Proteins Raf protein Rats Rats, Wistar Reactive oxygen species RKIP Sodium Sodium arsenite Sodium Compounds - toxicity Variance analysis antioxidant enzymes arsenic melatonin RKIP
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Arsenic (As) is a highly toxic metal and one of the main factors in cancer development through oxidative stress and production of reactive oxygen species. Prior research has demonstrated melatonin's potential as a free radical scavenger. Raf kinase inhibitory protein (RKIP) is an important regulator of intracellular signaling pathways that has been linked to various types of cancer. The aim of this research was to explore the influence of melatonin's antioxidant properties on the expression of the protein RKIP and the antioxidant status of liver tissue in rats that were exposed to arsenic. Thirty two male Wistar rats were divided into four groups of eight, including control, melatonin‐treated (20 mg/Kg of melatonin), sodium arsenite‐treated (5.5 mg/Kg of sodium arsenite), and melatonin + sodium arsenite‐treated groups (combination) for 4 weeks. The expression level of protein RKIP was measured by Western blot, and malondialdehyde (MDA) content of the liver as well as the activities of antioxidant enzymes were measured. The data analyzed using one‐way ANOVA (significance level of p < 0.05) and GraphPad Prism (9) software. Sodium arsenite treatment led to a significant decrease in RKIP protein expression and antioxidant enzyme activity, and an increase in liver MDA levels (p < 0.001). Conversely, melatonin treatment in the combination group resulted in a significant increase in RKIP protein expression and antioxidant enzyme activity and a decrease in liver MDA levels (p < 0.05). These findings suggest that melatonin can attenuate oxidative damage caused by arsenic in liver cells by enhancing RKIP protein expression and antioxidant enzyme activity. Melatonin can attenuate oxidative damage caused by arsenic in liver cells by enhancing RKIP protein expression and antioxidant enzyme activity.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1095-6670 1099-0461 1099-0461
DOI:	10.1002/jbt.23835