Scintigraphic imaging using technetium-99m-labeled ceftizoxime in an experimental model of acute osteomyelitis in rats

PURPOSETo investigate inflammatory (zymosan) and infectious (Staphylococcus aureus) processes in experimental models in rats using technetium-99m-labeled ceftizoxime (CFT). METHODSMale Wistar rats were used for the development of the inflammatory (zymosan) and infectious (S. aureus) processes in the...

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Published in:	Nuclear medicine communications Vol. 29; no. 9; pp. 830 - 836
Main Authors:	Diniz, Simone Odilia F, Rezende, Cleuza Maria F, Serakides, Rogéria, Ferreira, Raphael L.B, Ribeiro, Tatiana G, Martin-Comin, Josep, Cardoso, Valbert N
Format:	Journal Article
Language:	English
Published:	England Lippincott Williams & Wilkins, Inc 01-09-2008
Subjects:	Animals Blood Proteins - chemistry Ceftizoxime - analogs & derivatives Ceftizoxime - pharmacology Disease Models, Animal Inflammation Male Organotechnetium Compounds - pharmacology Osteomyelitis - diagnosis Osteomyelitis - diagnostic imaging Radionuclide Imaging - methods Radiopharmaceuticals - pharmacology Rats Rats, Wistar Sepsis - diagnosis Sepsis - diagnostic imaging Staphylococcal Infections - diagnosis Staphylococcal Infections - diagnostic imaging Time Factors Zymosan - metabolism
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Summary:	PURPOSETo investigate inflammatory (zymosan) and infectious (Staphylococcus aureus) processes in experimental models in rats using technetium-99m-labeled ceftizoxime (CFT). METHODSMale Wistar rats were used for the development of the inflammatory (zymosan) and infectious (S. aureus) processes in the medullary cavity of the left tibia. Sterile saline was used for the control group. At 48 h after induction of the processes, the animals were anesthetized and scintigraphic images were acquired at 1, 2, 4, and 6 h after intravenous injection of 0.1 ml of Tc–CFT (55 MBq). Quantitative analysis of the scintigraphic images was performed by counting the radioactivity in the regions of interest. Samples of tibia were taken for histopathological examination. RESULTSThe images showed that Tc–CFT presented higher tropism to infectious foci than with the inflammatory site. The average value of the target/nontarget ratio of the Tc–CFT was significantly higher in the infected (2.40±0.22) than in the inflamed tibia (1.50±0.05) and the control group (1.05±0.04) for all of the investigated times. The histological data showed a similar inflammatory response for both the S. aureus and zymosan groups. CONCLUSIONThe Tc–CFT presented a high tropism and retention for an infected region in this model of osteomyelitis, thereby constituting an interesting strategy to distinguish aseptic from septic sites.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-3636
DOI:	10.1097/MNM.0b013e3283000514