Sucrose Represses the Developmentally Controlled Transient Activation of the Plastocyanin Gene in Arabidopsis thaliana Seedlings

Sucrose Represses the Developmentally Controlled Transient Activation of the Plastocyanin Gene in Arabidopsis thaliana Seedlings

The plastocyanin (PC) gene of Arabidopsis thaliana is activated independently of light during early seedling development. In etiolated seedlings, PC mRNA levels increase transiently and a maximum dark level is reached after 2 d of growth in darkness. In etiolated transgenic seedlings carrying a chim...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) Vol. 110; no. 2; pp. 455 - 463
Main Authors: Dijkwel, Paul P., Patricia A. M. Kock, Rex Bezemer, Weisbeek, Peter J., Sjef C. M. Smeekens
Format: Journal Article
Language:English
Published: Rockville, MD American Society of Plant Physiologists 01-02-1996
Subjects:
Biological and medical sciences
Developmental biology
Fundamental and applied biological sciences. Psychology
Gene expression
Gene expression regulation
Gene Regulation and Molecular Genetics
Germination
Messenger RNA
Molecular and cellular biology
Molecular genetics
Plant cells
Plants
Repression
Seedlings
Sugars
Plastocyanin
Sucrose
Repression
Gene expression
Arabidopsis thaliana
Regulation(control)
Gene
Cruciferae
RNA synthesis
Dicotyledones
Light
Angiospermae
Plantlet
Carbohydrate
Spermatophyta
Transgenic plant
Developmental stage
Experimental plant
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The plastocyanin (PC) gene of Arabidopsis thaliana is activated independently of light during early seedling development. In etiolated seedlings, PC mRNA levels increase transiently and a maximum dark level is reached after 2 d of growth in darkness. In etiolated transgenic seedlings carrying a chimeric PC-promoter: luciferase fusion gene, luciferase activity is similarly increased after 2 d of growth. The transient increase in PC mRNA and luciferase activity levels can be repressed by sucrose. Nonmetabolizable sugars and polyethylene glycol do not have a major effect on PC gene expression. Also, light-grown seedlings show a similar transient and sucrose-sensitive increase in PC mRNA levels and luciferase activity, as in dark-grown seedlings, but here expression levels are 15-fold higher. These findings suggest the presence of a sucrose-sensitive, developmentally controlled expression mechanism that operates independently of light.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.110.2.455