Embryonic form of N-CAM and development of the rat corticospinal tract; immuno-electron microscopical localization during spinal white matter ingrowth

The neural cell adhesion molecule N-CAM has been proposed to function in the guidance of outgrowing axons in the peripheral and central nervous system. Light microscopic observations have shown that the embryonic form of N-CAM (200–230 kDa) is present in the ventralmost part of the dorsal funiculus...

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Published in:	Brain research. Developmental brain research Vol. 94; no. 1; pp. 99 - 105
Main Authors:	Joosten, E.A.J., Reshilov, L.N., Gispen, W.H., Bär, P.R.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 14-06-1996
Subjects:	Age Factors Animals Antibodies, Monoclonal Biomarkers Cell Adhesion Molecules, Neuronal - analysis Cell Adhesion Molecules, Neuronal - chemistry Cell Adhesion Molecules, Neuronal - immunology Cell Division - physiology Corticospinal tract Growth cone Guidance Immunohistochemistry Isomerism Microscopy, Immunoelectron Nerve Fibers - chemistry Nerve Fibers - ultrastructure Neural cell adhesion molecule Neurites - chemistry Neurites - ultrastructure p-Dimethylaminoazobenzene Pyramidal tract Pyramidal Tracts - chemistry Pyramidal Tracts - cytology Pyramidal Tracts - growth & development Rats Rats, Wistar Tract formation Guidance Pyramidal tract Tract formation Neural cell adhesion molecule L1 Corticospinal tract Growth cone
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Summary:	The neural cell adhesion molecule N-CAM has been proposed to function in the guidance of outgrowing axons in the peripheral and central nervous system. Light microscopic observations have shown that the embryonic form of N-CAM (200–230 kDa) is present in the ventralmost part of the dorsal funiculus during corticospinal tract (CST) ingrowth in the first postnatal week (Joosten, Dev. Brain Res., 78 (1994) 226–236). Here, the subcellular localization of the embryonic form of N-CAM (E-NCAM) is determined by pre-embedding staining on vibratone sections and by postembedding immunogold-labelling on Epon embedded spinal cord sections. The electron microscopical observations indicate that E-NCAM is present on the outer membrane of CST growth cones as well as other unmyelinated axons which are present in the ventralmost part of the dorsal funiculus. Furthermore, E-NCAM is localized in an irregular patchy way on the outer side of the axonal membrane of small unmyelinated, later arriving CST axons. From these results it may be deduced that E-NCAM is involved in CST tract formation through guidance of outgrowing pioneer CST growth cones along other unmyelinated axons and through mediation of axon fasciculation of later arriving CST axons.
ISSN:	0165-3806
DOI:	10.1016/0165-3806(96)00017-X