NMDA neurotoxicity in murine cortical cell cultures is not attenuated by hemoglobin or inhibition of nitric oxide synthesis

The role of nitric oxide in N-methyl-D-aspartate (NMDA) neurotoxicity was investigated in murine cortical cell cultures. Exposure of cultures to 300 microM NMDA for 5 min resulted in death of 50-80% of neurons over the subsequent 24 h. This injury was not attenuated by hemoglobin, the nitric oxide s...

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Bibliographic Details
Published in:	Neuroscience letters Vol. 153; no. 1; p. 53
Main Authors:	Regan, R F, Renn, K E, Panter, S S
Format:	Journal Article
Language:	English
Published:	Ireland 16-04-1993
Subjects:	Animals Cells, Cultured Cerebral Cortex - cytology Cerebral Cortex - drug effects Dizocilpine Maleate - pharmacology Hemoglobins - pharmacology Mice N-Methylaspartate - toxicity Nerve Degeneration Neurons - drug effects Neurotoxins - pharmacology Nitric Oxide - antagonists & inhibitors Nitric Oxide - metabolism
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Summary:	The role of nitric oxide in N-methyl-D-aspartate (NMDA) neurotoxicity was investigated in murine cortical cell cultures. Exposure of cultures to 300 microM NMDA for 5 min resulted in death of 50-80% of neurons over the subsequent 24 h. This injury was not attenuated by hemoglobin, the nitric oxide synthase (NOS) inhibitors NG monomethyl-L-arginine (MMA) or N omega-nitro-L-arginine (NA), or L-arginine depletion. Hemoglobin and NOS inhibitors consistently prevented the increase in cyclic guanosine monophosphate (cGMP) seen after NMDA exposure. These results suggest that NMDA neurotoxicity in this cell culture system is mediated, at least in part, by mechanisms other than NOS activation.
ISSN:	0304-3940
DOI:	10.1016/0304-3940(93)90075-V