Identification and quantification of gold engineered nanomaterials and impaired fluid transfer across the rat placenta via ex vivo perfusion

Identification and quantification of gold engineered nanomaterials and impaired fluid transfer across the rat placenta via ex vivo perfusion

•Morphological barriers remain intact using our ex vivo placental perfusion technique.•Gold nanoparticles can translocate across the placenta within 20 min of material infusion.•Nanoparticle infusion can impair fluid flow from the maternal to fetal compartment. Development and implementation of prod...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy Vol. 117; p. 109148
Main Authors: D’Errico, J.N., Doherty, C., Fournier, S.B., Renkel, N., Kallontzi, S., Goedken, M., Fabris, L., Buckley, B., Stapleton, P.A.
Format: Journal Article
Language:English
Published: France Elsevier Masson SAS 01-09-2019
Elsevier
Subjects:
Animals
Engineered nanomaterial
Female
Gold - pharmacology
Gold nanomaterials
ICP-MS
Nanostructures - chemistry
Organ perfusion
Perfusion
Placenta
Placenta - anatomy & histology
Placenta - drug effects
Placenta - metabolism
Pregnancy
Rats, Sprague-Dawley
Engineered nanomaterial
Placenta
ICP-MS
Organ perfusion
Gold nanomaterials
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Summary:•Morphological barriers remain intact using our ex vivo placental perfusion technique.•Gold nanoparticles can translocate across the placenta within 20 min of material infusion.•Nanoparticle infusion can impair fluid flow from the maternal to fetal compartment. Development and implementation of products incorporating nanoparticles are occurring at a rapid pace. These particles are widely utilized in domestic, occupational, and biomedical applications. Currently, it is unclear if pregnant women will be able to take advantage of the potential biomedical nanoproducts out of concerns associated with placental transfer and fetal interactions. We recently developed an ex vivo rat placental perfusion technique to allow for the evaluation of xenobiotic transfer and placental physiological perturbations. In this study, a segment of the uterine horn and associated placenta was isolated from pregnant (gestational day 20) Sprague-Dawley rats and placed into a modified pressure myography vessel chamber. The proximal and distal ends of the maternal uterine artery and the vessels of the umbilical cord were cannulated, secured, and perfused with physiological salt solution (PSS). The proximal uterine artery and umbilical artery were pressurized at 80 mmHg and 50 mmHg, respectively, to allow countercurrent flow through the placenta. After equilibration, a single 900 μL bolus dose of 20 nm gold engineered nanoparticles (Au-ENM) was introduced into the proximal maternal artery. Distal uterine and umbilical vein effluents were collected every 10 min for 180 min to measure placental fluid dynamics. The quantification of Au-ENM transfer was conducted via inductively coupled plasma mass spectrometry (ICP-MS). Overall, we were able to measure Au-ENM within uterine and umbilical effluent with 20 min of material infusion. This novel methodology may be widely incorporated into studies of pharmacology, toxicology, and placental physiology.
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2019.109148