Interlineage distribution and characteristics of the structure of two subfamilies of Drosophila melanogaster MDG4 (gypsy) retrotransposon

The distribution of two variants of MDG4 (gypsy) was analyzed in several Drosophila melanogaster strains. Southern blot hybridization revealed the inactive variant of MDG4 in all strains examined and active MDG4 only in some of them. Most of the strains harboring the active MDG4 variant were recentl...

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Bibliographic Details
Published in:	Genetika Vol. 37; no. 2; p. 175
Main Authors:	Razorenova, O V, Karpova, N N, Smirnova, Iu B, Kusulidu, L K, Reneva, N K, Subocheva, E A, Kim, A I, Liubomirskaia, N V, Il'in, Iu V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-02-2001
Subjects:	Animals Autoradiography Blotting, Southern Drosophila melanogaster - genetics In Situ Hybridization Retroelements
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Summary:	The distribution of two variants of MDG4 (gypsy) was analyzed in several Drosophila melanogaster strains. Southern blot hybridization revealed the inactive variant of MDG4 in all strains examined and active MDG4 only in some of them. Most of the strains harboring the active MDG4 variant were recently isolated from natural populations. It is of interest that the active MDG4 prevailed over the inactive one only in strains carrying the mutant flamenco gene. Several lines were analyzed in more detail. The number of MDG4 sites on salivary-gland polytene chromosomes was established via in situ hybridization, and MDG4 was tested for transposition using the ovoD test.
ISSN:	0016-6758