A willow UDP-glycosyltransferase involved in salicinoid biosynthesis

A willow UDP-glycosyltransferase involved in salicinoid biosynthesis

The salicinoids are phenolic glycosides that are characteristic secondary metabolites of the Salicaceae, particularly willows and poplars. Despite the well-known pharmacology of salicin, that led to the development of aspirin >100 years ago, the biosynthetic pathways leading to salicinoids have y...

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Bibliographic Details
Published in:Journal of experimental botany Vol. 72; no. 5; pp. 1634 - 1648
Main Authors: Kulasekaran, Satish, Cerezo-Medina, Sergio, Harflett, Claudia, Lomax, Charlotte, de Jong, Femke, Rendour, Amelie, Ruvo, Gianluca, Hanley, Steven J, Beale, Michael H, Ward, Jane L
Format: Journal Article
Language:English
Published: England 27-02-2021
Subjects:
Glycosides - biosynthesis
Glycosyltransferases - genetics
Plants, Genetically Modified - enzymology
Populus - genetics
Salix - enzymology
Salix - genetics
Uridine Diphosphate
tremulacin
Salix
UGT
salicortin
salicyl-7-benzoate
Poplar
salicin
salicinoid
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Summary:The salicinoids are phenolic glycosides that are characteristic secondary metabolites of the Salicaceae, particularly willows and poplars. Despite the well-known pharmacology of salicin, that led to the development of aspirin >100 years ago, the biosynthetic pathways leading to salicinoids have yet to be defined. Here, we describe the identification, cloning, and biochemical characterization of SpUGT71L2 and SpUGT71L3-isozymic glycosyltransferases from Salix purpurea-that function in the glucosylation of ortho-substituted phenols. The best substrate in vitro was salicyl-7-benzoate. Its product, salicyl-7-benzoate glucoside, was shown to be endogenous in poplar and willow. Together they are inferred to be early intermediates in the biosynthesis of salicortin and related metabolites in planta. The role of this UDP-glycosyltransferase was confirmed via the metabolomic analysis of transgenic plants produced by RNAi knockdown of the poplar orthologue (UGT71L1) in the hybrid clone Populus tremula×P. alba, INRA 717-1B4.
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ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/eraa562