Search Results - "Remaut, K."

Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer by Santin, Yoann G., Sogues, Adrià, Bourigault, Yvann, Remaut, Han K., Laloux, Géraldine

“…Predatory bacteria feed upon other bacteria in various environments. Bdellovibrio exovorus is an obligate epibiotic predator that attaches on the prey cell…”
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Hyaluronic acid coating of gold nanoparticles for intraocular drug delivery: Evaluation of the surface properties and effect on their distribution by Apaolaza, P.S., Busch, M., Asin-Prieto, E., Peynshaert, K., Rathod, R., Remaut, K., Dünker, N., Göpferich, A.

“…Due to the unique anatomical structure of the eye, ocular drug delivery is a promising delivery route for the treatment of several ocular diseases, such as the…”
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Fluorescent Labeling of Plasmid DNA and mRNA: Gains and Losses of Current Labeling Strategies by Rombouts, K, Braeckmans, K, Remaut, K

“…Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be…”
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PEGylation of biodegradable dextran nanogels for siRNA delivery by Naeye, B., Raemdonck, K., Remaut, K., Sproat, B., Demeester, J., De Smedt, S.C.

“…Delivering intact small interfering RNA (siRNA) into the cytoplasm of targeted cells in vivo is considered a major obstacle in the development of clinically…”
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Cell division responsive peptides for optimized plasmid DNA delivery: The mitotic window of opportunity? by Remaut, K., Symens, N., Lucas, B., Demeester, J., De Smedt, S.C.

“…The delivery of plasmid DNA remains hard to achieve, especially due to the presence of the nuclear membrane barrier. During cell division, however, the nuclear…”
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Therapeutic potential of non‐viral MRNA delivery to Müller cells for neuroprotection by Devoldere, J., Peynshaert, K., De Smedt, S., Remaut, K.

“…Purpose Retinal cell degeneration is a leading cause of vision loss in many ocular diseases. The progressive loss of retinal cells is often hereditary, but…”
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Pegylation of liposomes favours the endosomal degradation of the delivered phosphodiester oligonucleotides by Remaut, K., Lucas, B., Braeckmans, K., Demeester, J., De Smedt, S.C.

“…Liposomal vesicles have been widely investigated as carriers for the intracellular delivery of oligonucleotides (ONs). To avoid unspecific uptake by the…”
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Toward rational design of gene carriers: a novel ex vivo model to study the vitreoretinal interface as a barrier by Peynshaert, K., Fradot, V., Picaud, S., De Smedt, S., Remaut, K.

“…Purpose The vitreoretinal interface poses a serious hurdle for the retinal delivery of viral and non‐viral gene vectors after intravitreal injection. We have…”
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Intravitreal mobility of nanoparticles: how to make a move toward successful ocular gene delivery? by Remaut, K., Devoldere, J., Peynshaert, K., Martens, T., Engbersen, J., Braeckmans, K., De Smedt, S.

“…Summary Intravitreal injection is an interesting delivery route for the administration of nanoparticles for treatment of a variety of retinopathies. To reach…”
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Protection of Oligonucleotides against Enzymatic Degradation by Pegylated and Nonpegylated Branched Polyethyleneimine by Remaut, K, Lucas, B, Raemdonck, K, Braeckmans, K, Demeester, J, De Smedt, S. C

“…Among the cationic polymers, polyethyleneimine (PEI) is a promising candidate for delivery of oligodeoxynucleotides (ODNs). In this study, we wondered whether…”
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Matrix systems for siRNA delivery by Naeye, B, Raemdonck, K, Remaut, K, Demeester, J, De Smedt, S C

“…Over the last decade, considerable effort has been put in the implementation of RNA interference (RNAi) as a treatment for various disorders. As RNAi occurs in…”
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Efficient delivery of intact phosphodiester oligonucleotides by poly-β-amino esters by Remaut, K., Symens, N., Lucas, B., Demeester, J., De Smedt, S.C.

“…Due to their great instability, phosphodiester antisense oligonucleotides (PO-ODNs) are rapidly degraded in the intracellular environment, which limits their…”
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Nuclear inclusion of inert and chromatin-targeted polystyrene spheres and plasmid DNA containing nanoparticles by Symens, N., Walzack, R., Demeester, J., Mattaj, I., De Smedt, S.C., Remaut, K.

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Can we better understand the intracellular behavior of DNA nanoparticles by fluorescence correlation spectroscopy? by Remaut, K., Lucas, B., Raemdonck, K., Braeckmans, K., Demeester, J., De Smedt, S.C.

“…The use of non-viral gene carriers to deliver small nucleic acids like antisense oligonucleotides (ODNs) and small interfering RNA (siRNA) remains an…”
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FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery by Remaut, K., Lucas, B., Braeckmans, K., Sanders, N.N., De Smedt, S.C., Demeester, J.

“…The intracellular degradation of single-stranded, double-labeled oligonucleotides (ONs) was studied by following the disappearance of Fluorescence Resonance…”
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Studying biophysical barriers to DNA delivery by advanced light microscopy by De Smedt, S.C., Remaut, K., Lucas, B., Braeckmans, K., Sanders, N.N., Demeester, J.

“…Advanced light microscopy (ALM) has been intensively employed by biophysicists to reveal cellular mechanisms. As described in this review, ALM clearly has…”
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Studying the Intracellular Dissociation of Polymer−Oligonucleotide Complexes by Dual Color Fluorescence Fluctuation Spectroscopy and Confocal Imaging by Lucas, B, Remaut, K, Sanders, N. N, Braeckmans, K, De Smedt, S. C, Demeester, J

“…To transfect cells, cationic polymers as well as cationic liposomes are widely investigated as carriers for both oligonucleotides and plasmid DNA. A major step…”
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Protection of oligonucleotides against nucleases by pegylated and non-pegylated liposomes as studied by fluorescence correlation spectroscopy by Remaut, K., Lucas, B., Braeckmans, K., Sanders, N.N., Demeester, J., De Smedt, S.C.

“…Antisense phosphodiester oligonucleotides (ONs), complexed to carriers such as cationic liposomes, inhibit the production of proteins. The biochemical and…”
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Delivery of Phosphodiester Oligonucleotides:  Can DOTAP/DOPE Liposomes Do the Trick? by Remaut, K, Lucas, B, Braeckmans, K, Sanders, N. N, Demeester, J, De Smedt, S. C

“…Delivering phosphodiester ONs (PO-ONs) remains an attractive but challenging goal in antisense therapy. Both in the literature and in our experiments, most…”
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Towards a better understanding of the dissociation behavior of liposome-oligonucleotide complexes in the cytosol of cells by Lucas, B., Remaut, K., Sanders, N.N., Braeckmans, K., De Smedt, S.C., Demeester, J.

“…To obtain real breakthroughs in antisense therapy, it is necessary to understand the cellular behavior of antisense delivery systems. Fluorescence fluctuation…”
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