Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay

Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide...

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Published in:	Journal of microbiological methods Vol. 88; no. 2; pp. 255 - 262
Main Authors:	Sabagh, Bruna Peres, Souto, Aline da Silva Soares, Reis, Louise Moreira, da Silva, Sérgio Alves, Pereira, Daniella Cristina Rodrigues, Neves, Marta de Campos, Pinheiro, Rodrigo Rollin, Duarte, Rafael Silva, Miyazaki, Neide Hiromi Tokumaru, Villas Bôas, Maria Helena Simões
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01-02-2012 Elsevier
Subjects:	ADC enrichment Animals Biological and medical sciences Brazil Cattle chemists culture media Culture Media - chemistry Disinfectant assay disinfectants Disinfectants - pharmacology fetal bovine serum Fundamental and applied biological sciences. Psychology glucose Glucose - pharmacology Glutaral - pharmacology Glutaraldehyde in vitro studies laws and regulations medical equipment Microbial Sensitivity Tests - methods Microbiology Mycobacteria Mycobactericidal susceptibility assay Mycobacterium - drug effects Mycobacterium - growth & development Mycobacterium abscessus Mycobacterium bovis BCG Mycobacterium chelonae Mycobacterium fortuitum Serum fetal bovine enrichment statistical analysis Statistics, Nonparametric sterilizing Brazil ADC MS Mycobacteria ADC enrichment 7H9 INCQS K AOAC ANVISA FBSG PB CFU Glutaraldehyde Mycobactericidal susceptibility assay Disinfectant assay SGM RGM Serum fetal bovine enrichment FIOCRUZ Mycobacterium Bovine Vertebrata Sensitivity Mammalia Mycobacteriales Efficiency Mycobacteriaceae Bacteria Serum Actinomycetes Artiodactyla Veterinary Ungulata Comparative study
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Summary:	Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficacy of sterilizing and disinfectant products, used for semi critical medical devices, against the Mycobacterium bovis BCG Moreau and Mycobacterium abscessus subsp. bolletii INCQS 00594 strains by using the Confirmative in vitro Test for Determining Tuberculocidal Activity of Disinfectants recommended by the Association of Official Analytical Chemists. These changes have caused additional costs and increased problems for importation of enrichment products at national laboratories where disinfectant efficacy assay service is performed. Middlebrook ADC Enrichment (ADC) is provided by a unique manufacturer and used in the official protocol. The aim of the present study was to evaluate an alternative in house low-cost enrichment composed of fetal bovine serum and glucose (FBSG) with ADC for performance of disinfectant efficacy assay against mycobacteria. After obtaining the growth curves for M. abscessus ATCC 19977, M. abscessus subsp. bolletii INCQS 00594, Mycobacterium chelonae ATCC 35752, and Mycobacterium fortuitum ATCC 6841 by using ADC enrichment and FBSG in Kirchners and 7H9 culture media. Through statistical analysis via the Kruskal–Wallis test on the evaluation of microorganism growth rate, it was observed that there was no inhibition of RGM growth by any of the enrichments used. These results suggest that low-cost enrichment FBSG may be used as a potential substitute of ADC for composition of media for mycobacterial growth, including in disinfectant tests. ► We evaluate an alternative enrichment (FBSG) for disinfectant efficacy assay. ► There was no interference in RGM growth by any of the enrichments used. ► The FBSG may be used as a potential substitute of ADC for mycobacterial growth.
Bibliography:	http://dx.doi.org/10.1016/j.mimet.2011.12.004 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2011.12.004